一、彭氏变形杆菌菌株简介
1、菌株名称：彭氏变形杆菌 Proteus penneri
2、菌株编号：TS276307
3、其他保藏编号：ATCC 33519
4、彭氏变形杆菌菌株分离源：尿液
二、彭氏变形杆菌储存条件
冻干菌种和试管斜面请置于2-8℃冷藏。甘油菌菌种请置于-80℃冷冻。
三、彭氏变形杆菌培养条件
1、培养基：营养肉汁琼脂培养基（NA）；胰蛋白胨大豆肉汤培养基（TSA）；LB培养基；
2、培养温度：37℃
3、培养时间：18~24小时
四、彭氏变形杆菌注意事项
1、冻干首次活化，干粉要全部用完，不能保留，用0.1-0.2ml的培养液或者无菌水溶解，接种在2支斜面上，因冻干菌处于休眠状态，请勿接种多支斜面或平板，以免因接种量不足而导致复苏不成功。
2、复苏后，微生物菌种应保藏于建议的温度、清洁和干燥的地方，室温放置时间过长会导致菌种衰退。
3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物不负责任。
4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。
Proteus penneri is a Gram-negative, facultatively anaerobic, rod-shaped bacterium.[1] It is an invasive pathogen[2] and a cause of nosocomial infections of the urinary tract or open wounds.[3] Pathogens have been isolated mainly from the urine of patients with abnormalities in the urinary tract, and from stool.[4] P. penneri strains are naturally resistant to numerous antibiotics, including penicillin G, amoxicillin, cephalosporins, oxacillin, and most macrolides, but are naturally sensitive to aminoglycosides, carbapenems, aztreonam, quinolones, sulphamethoxazole, and co-trimoxazole. Isolates of P. penneri have been found to be multiple drug-resistant (MDR) with resistance to six to eight drugs. β-lactamase production has also been identified in some isolates.[5]
The Proteus penneri group of bacteria was named in 1982. It reclassified a group of strains formerly known as Proteus vulgaris biogroup 1.[6] In 1978, Brenner et al. showed through DNA hybridization studies that P. vulgaris was a heterogenous species.[7] In 1981, Hickman et al conducted experiments on 20 indole-negative strains previously grouped with P.vulgaris and demonstrated the existence of three P. vulgaris biogroups. P. vulgaris biogroup 1, or indole-negative P. vulgaris, was distinguished as a new species within the genus Proteus in 1982.[1] The new species was named Proteus penneri in honor of John Penner, a Canadian microbiologist.[4]
一、彭氏变形杆菌菌株简介
1、菌株名称：彭氏变形杆菌 Proteus penneri
2、菌株编号：TS276307
3、其他保藏编号：ATCC 33519
4、彭氏变形杆菌菌株分离源：尿液
二、彭氏变形杆菌储存条件
冻干菌种和试管斜面请置于2-8℃冷藏。甘油菌菌种请置于-80℃冷冻。
三、彭氏变形杆菌培养条件
1、培养基：营养肉汁琼脂培养基（NA）；胰蛋白胨大豆肉汤培养基（TSA）；LB培养基；
2、培养温度：37℃
3、培养时间：18~24小时
四、彭氏变形杆菌注意事项
1、冻干首次活化，干粉要全部用完，不能保留，用0.1-0.2ml的培养液或者无菌水溶解，接种在2支斜面上，因冻干菌处于休眠状态，请勿接种多支斜面或平板，以免因接种量不足而导致复苏不成功。
2、复苏后，微生物菌种应保藏于建议的温度、清洁和干燥的地方，室温放置时间过长会导致菌种衰退。
3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种不活等损失，泰斯拓生物不负责任。
4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。
Proteus penneri is a Gram-negative, facultatively anaerobic, rod-shaped bacterium.[1] It is an invasive pathogen[2] and a cause of nosocomial infections of the urinary tract or open wounds.[3] Pathogens have been isolated mainly from the urine of patients with abnormalities in the urinary tract, and from stool.[4] P. penneri strains are naturally resistant to numerous antibiotics, including penicillin G, amoxicillin, cephalosporins, oxacillin, and most macrolides, but are naturally sensitive to aminoglycosides, carbapenems, aztreonam, quinolones, sulphamethoxazole, and co-trimoxazole. Isolates of P. penneri have been found to be multiple drug-resistant (MDR) with resistance to six to eight drugs. β-lactamase production has also been identified in some isolates.[5]
The Proteus penneri group of bacteria was named in 1982. It reclassified a group of strains formerly known as Proteus vulgaris biogroup 1.[6] In 1978, Brenner et al. showed through DNA hybridization studies that P. vulgaris was a heterogenous species.[7] In 1981, Hickman et al conducted experiments on 20 indole-negative strains previously grouped with P.vulgaris and demonstrated the existence of three P. vulgaris biogroups. P. vulgaris biogroup 1, or indole-negative P. vulgaris, was distinguished as a new species within the genus Proteus in 1982.[1] The new species was named Proteus penneri in honor of John Penner, a Canadian microbiologist.[4]
