1、菌株名称：金黄色葡萄球菌RN4220 Staphylococcus aureus
3、菌株来源: 金黄色葡萄球菌RN4220菌株，是利用紫外线UV和化学方法，诱导NCTC8325菌株产生的突变株。
斜面菌种和冻干菌种应在2~8℃保存。
1、培养基编号： （1）MH 培养基 ，（2）TSB培养基 ，（3）Brain heart infusion Broth培养基 以上建议的培养基三选一
2、培养温度：37 ℃, 有氧；
3、培养时间：24—48h。
1、RN4220菌株一直以来被广泛的应用于金葡菌基因研究。该菌株来源于NCTC8325-4，是一株限制性内切酶缺陷菌株，能够接受来源于外部的其他物种的DNA质粒。RN4220体内没有其他质粒，对大部分抗生素都敏感。金葡菌RN4220是用来转化大肠杆菌质粒DNA的金黄色葡萄球菌菌株。该菌株在基因sau1 hsdR上有突变，该突变的产生可以导致RN4220成为限制修饰系统基因缺陷型，可以用来作为大肠杆菌质粒DNA和金黄色葡萄球菌质粒转化的中间宿主菌。该菌株基因型是mec阴性，rsbU阴性，agr阴性。MLST测序分型为8，e基因组SPA分型为59，e基因组SPA重复序列为YHGGFMBQBLO。 Ridom SPA分型为t211。RN4220的基因组序列已经测序成功，Genbank编号为AFGU00000000.，RN4220属于甲氧西林敏感型金黄色葡萄球菌（MSSA）。
2、复苏后，微生物菌种应保藏于建议的温度、清洁和干燥的地方，室温放置时间过长会导致菌种衰退。
3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种的复苏不活损失，泰斯拓生物不负责任。
4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。
Staphylococcus aureus is a Gram-positive round-shaped bacterium, a member of the Bacillota, and is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. It is often positive for catalase and nitrate reduction and is a facultative anaerobe that can grow without the need for oxygen.[1] Although S. aureus usually acts as a commensal of the human microbiota it can also become an opportunistic pathogen, being a common cause of skin infections including abscesses, respiratory infections such as sinusitis, and food poisoning. Pathogenic strains often promote infections by producing virulence factors such as potent protein toxins, and the expression of a cell-surface protein that binds and inactivates antibodies. S. aureus is one of the leading pathogens for deaths associated with antimicrobial resistance and the emergence of antibiotic-resistant strains such as methicillin-resistant S. aureus (MRSA) is a worldwide problem in clinical medicine. Despite much research and development, no vaccine for S. aureus has been approved.
An estimated 20% to 30% of the human population are long-term carriers of S. aureus[2][3] which can be found as part of the normal skin flora, in the nostrils,and as a normal inhabitant of the lower reproductive tract of women.[5][6] S. aureus can cause a range of illnesses, from minor skin infections, such as pimples,[7] impetigo, boils, cellulitis, folliculitis, carbuncles, scalded skin syndrome, and abscesses, to life-threatening diseases such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome, bacteremia, and sepsis. It is still one of the five most common causes of hospital-acquired infections and is often the cause of wound infections following surgery. Each year, around 500,000 patients in hospitals of the United States contract a staphylococcal infection, chiefly by S. aureus.[8] Up to 50,000 deaths each year in the United States are linked with S. aureus infections.
1、菌株名称：金黄色葡萄球菌RN4220 Staphylococcus aureus
3、菌株来源: 金黄色葡萄球菌RN4220菌株，是利用紫外线UV和化学方法，诱导NCTC8325菌株产生的突变株。
斜面菌种和冻干菌种应在2~8℃保存。
1、培养基编号： （1）MH 培养基 ，（2）TSB培养基 ，（3）Brain heart infusion Broth培养基 以上建议的培养基三选一
2、培养温度：37 ℃, 有氧；
3、培养时间：24—48h。
1、RN4220菌株一直以来被广泛的应用于金葡菌基因研究。该菌株来源于NCTC8325-4，是一株限制性内切酶缺陷菌株，能够接受来源于外部的其他物种的DNA质粒。RN4220体内没有其他质粒，对大部分抗生素都敏感。金葡菌RN4220是用来转化大肠杆菌质粒DNA的金黄色葡萄球菌菌株。该菌株在基因sau1 hsdR上有突变，该突变的产生可以导致RN4220成为限制修饰系统基因缺陷型，可以用来作为大肠杆菌质粒DNA和金黄色葡萄球菌质粒转化的中间宿主菌。该菌株基因型是mec阴性，rsbU阴性，agr阴性。MLST测序分型为8，e基因组SPA分型为59，e基因组SPA重复序列为YHGGFMBQBLO。 Ridom SPA分型为t211。RN4220的基因组序列已经测序成功，Genbank编号为AFGU00000000.，RN4220属于甲氧西林敏感型金黄色葡萄球菌（MSSA）。
2、复苏后，微生物菌种应保藏于建议的温度、清洁和干燥的地方，室温放置时间过长会导致菌种衰退。
3、初次使用时请按照本说明书推荐条件进行复活培养，如使用其它类型培养基或培养条件造成菌种的复苏不活损失，泰斯拓生物不负责任。
4、使用者应保证菌种的安全存储和操作，带菌废弃物应高压灭菌处理后丢弃。
Staphylococcus aureus is a Gram-positive round-shaped bacterium, a member of the Bacillota, and is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. It is often positive for catalase and nitrate reduction and is a facultative anaerobe that can grow without the need for oxygen.[1] Although S. aureus usually acts as a commensal of the human microbiota it can also become an opportunistic pathogen, being a common cause of skin infections including abscesses, respiratory infections such as sinusitis, and food poisoning. Pathogenic strains often promote infections by producing virulence factors such as potent protein toxins, and the expression of a cell-surface protein that binds and inactivates antibodies. S. aureus is one of the leading pathogens for deaths associated with antimicrobial resistance and the emergence of antibiotic-resistant strains such as methicillin-resistant S. aureus (MRSA) is a worldwide problem in clinical medicine. Despite much research and development, no vaccine for S. aureus has been approved.
An estimated 20% to 30% of the human population are long-term carriers of S. aureus[2][3] which can be found as part of the normal skin flora, in the nostrils,and as a normal inhabitant of the lower reproductive tract of women.[5][6] S. aureus can cause a range of illnesses, from minor skin infections, such as pimples,[7] impetigo, boils, cellulitis, folliculitis, carbuncles, scalded skin syndrome, and abscesses, to life-threatening diseases such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome, bacteremia, and sepsis. It is still one of the five most common causes of hospital-acquired infections and is often the cause of wound infections following surgery. Each year, around 500,000 patients in hospitals of the United States contract a staphylococcal infection, chiefly by S. aureus.[8] Up to 50,000 deaths each year in the United States are linked with S. aureus infections.
