1、菌株名称：Pedobacter panaciterrae 人参地土地杆菌
2、菌株编号：TS278907
3、其它编号：KACC 12905= KCTC12594 = LMG 23400
4、具体用途：分类；研究；教学
斜面菌种和冻干菌种应在2~8℃保存。
1、培养基编号：R2A
2、培养温度：30℃
附: -80℃和液氮保藏方法:
(1)培养:将菌种接种于合适培养基，培养基用固体或液体均可，对生长较快(如大部分弧菌、希瓦氏菌)和生长较慢的菌株用液体培养基培养比较好。培养时期掌握在对数生长期或对数生长后期。
(2)收集菌体:液体培养时，离心弃去上清液；以平板或斜面培养时，刮取菌体。
(3)保护剂:20%甘油(或20%甘油+5%海藻糖保藏效果更好)灭菌。
(4)分装:将菌体与保护剂混匀，菌体用量不能太少，混匀后看上去要浑浊；分装到若干冻存管。
(5)降温:及时放冰箱，先放-20℃，1-2小时后转移至-70℃至-80℃。如放入液氨中保藏，须注意样品温度降至-40℃以下再放。
(6)活化: 从冰箱或液氮中取出甘油管后，立即放置在40C温水中摇晃使冰融化，约需50秒-100秒；吸取适量内容物至适宜培养基培养。
*也可从冰箱或液氮中取出后，用低温冰盒保温，牙签刮取少许冰接种至合适培养基，甘油管可再放回原处低温保藏(注意动作要快避免过度升温)。
•冻干首次活化, 干粉要全部用完，不能保留，用0.2-0.5m1的培养液或者无菌水溶解,接种在2支斜面上，因冻干菌种处于休眠状态,请勿接种多支斜面或平板，以免因接种不足而导致复苏不成功；
•复苏后，微生物菌种应保藏于建议的温度、清洁和千燥的地方，室温放置时间过长会导致菌种衰退;
•菌种操作应在无菌条件下进行;转种完毕,应经灭菌再做丢弃处理，以免污染周围环境。
•冻干菌复苏后，应根据菌种状况及时转接传代;
•操作前，如果有不明白之处，应先咨询我中心技术人员，避免不必要的损失。
A Gram-negative, facultatively anaerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 042T, was isolated from a soil sample of a ginseng field in Pocheon province (South Korea) and was characterized taxonomically by using a polyphasic approach. The isolate was positive for catalase and oxidase, but negative for gelatinase and production of indole and H2S. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were summed feature 4 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH; 35.7 %), iso-C(15 : 0) (24.3 %) and iso-C(17 : 0) 3-OH (11.6 %). Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 042T fell within the radiation of the cluster comprising Pedobacter species. On the basis of 16S rRNA gene sequence similarities, the phylogenetically closest recognized species of the genus Pedobacter to strain Gsoil 042T included Pedobacter heparinus DSM 2366T (98.1 %), Pedobacter africanus DSM 12126(T) (97.8 %) and Pedobacter caeni LMG 22862T (97.2 %). The 16S rRNA gene sequence similarity with other recognized species of the genus Pedobacter was less than 96.1 %. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness between strain Gsoil 042T and its phylogenetically closest neighbours was less than 43 %. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 042T should be classified as representing a novel species in the genus Pedobacter, for which the name Pedobacter panaciterrae sp. nov. is proposed. The type strain is Gsoil 042T (=KCTC 12594T=LMG 23400T).
1、菌株名称：Pedobacter panaciterrae 人参地土地杆菌
2、菌株编号：TS278907
3、其它编号：KACC 12905= KCTC12594 = LMG 23400
4、具体用途：分类；研究；教学
斜面菌种和冻干菌种应在2~8℃保存。
1、培养基编号：R2A
2、培养温度：30℃
附: -80℃和液氮保藏方法:
(1)培养:将菌种接种于合适培养基，培养基用固体或液体均可，对生长较快(如大部分弧菌、希瓦氏菌)和生长较慢的菌株用液体培养基培养比较好。培养时期掌握在对数生长期或对数生长后期。
(2)收集菌体:液体培养时，离心弃去上清液；以平板或斜面培养时，刮取菌体。
(3)保护剂:20%甘油(或20%甘油+5%海藻糖保藏效果更好)灭菌。
(4)分装:将菌体与保护剂混匀，菌体用量不能太少，混匀后看上去要浑浊；分装到若干冻存管。
(5)降温:及时放冰箱，先放-20℃，1-2小时后转移至-70℃至-80℃。如放入液氨中保藏，须注意样品温度降至-40℃以下再放。
(6)活化: 从冰箱或液氮中取出甘油管后，立即放置在40C温水中摇晃使冰融化，约需50秒-100秒；吸取适量内容物至适宜培养基培养。
*也可从冰箱或液氮中取出后，用低温冰盒保温，牙签刮取少许冰接种至合适培养基，甘油管可再放回原处低温保藏(注意动作要快避免过度升温)。
•冻干首次活化, 干粉要全部用完，不能保留，用0.2-0.5m1的培养液或者无菌水溶解,接种在2支斜面上，因冻干菌种处于休眠状态,请勿接种多支斜面或平板，以免因接种不足而导致复苏不成功；
•复苏后，微生物菌种应保藏于建议的温度、清洁和千燥的地方，室温放置时间过长会导致菌种衰退;
•菌种操作应在无菌条件下进行;转种完毕,应经灭菌再做丢弃处理，以免污染周围环境。
•冻干菌复苏后，应根据菌种状况及时转接传代;
•操作前，如果有不明白之处，应先咨询我中心技术人员，避免不必要的损失。
A Gram-negative, facultatively anaerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 042T, was isolated from a soil sample of a ginseng field in Pocheon province (South Korea) and was characterized taxonomically by using a polyphasic approach. The isolate was positive for catalase and oxidase, but negative for gelatinase and production of indole and H2S. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were summed feature 4 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH; 35.7 %), iso-C(15 : 0) (24.3 %) and iso-C(17 : 0) 3-OH (11.6 %). Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 042T fell within the radiation of the cluster comprising Pedobacter species. On the basis of 16S rRNA gene sequence similarities, the phylogenetically closest recognized species of the genus Pedobacter to strain Gsoil 042T included Pedobacter heparinus DSM 2366T (98.1 %), Pedobacter africanus DSM 12126(T) (97.8 %) and Pedobacter caeni LMG 22862T (97.2 %). The 16S rRNA gene sequence similarity with other recognized species of the genus Pedobacter was less than 96.1 %. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness between strain Gsoil 042T and its phylogenetically closest neighbours was less than 43 %. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 042T should be classified as representing a novel species in the genus Pedobacter, for which the name Pedobacter panaciterrae sp. nov. is proposed. The type strain is Gsoil 042T (=KCTC 12594T=LMG 23400T).
