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Recombinant human FEN1 protein
Recombinant human FEN1 protein
规格:
货期:
编号:TSENZ2275
品牌:Testobio
基因名 FEN1
英文全称 flap structure-specific endonuclease 1
别称 DNase IV, FEN 1, FEN1, Flap endonuclease 1, flap structure endonuclease 1, hFEN 1, Maturation factor 1, MF1, RAD2
中文名称 瓣结构特异性核酸内切酶1
Uniprot P39748
GeneID 2237
交叉反应(推测) Human Mouse Rat (Bovine Zebrafish Fruit fly Chicken Sheep)
氨基酸大小 43kDa
表达氨基酸 1-380aa
Background The protein encoded by this gene removes 5 overhanging flaps in DNA repair and processes the 5 ends of Okazaki fragments in lagging strand DNA synthesis.Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another.The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication.DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5 end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene.Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Application Immunology research
Derived from E.coli
Purification NI-NTA affinity purification
Formulation Lyophilized from a 0.2 μm filtered solution of Tris-HCl, PH8.0.
Shipping The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years.
Reconstitution Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Purity Purity≥85% as determined by reducing SDS-PAGE.
规格 10ug 50ug 100ug 1mg
合作单位: