phgH6 [hGH6]-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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phgH6 [hGH6]
phgH6 [hGH6]
规格:
货期:
编号:TS147921
品牌:Testobio
产品名称: phgH6 hGH6
商品货号: TS147921
Designations: phgH6 hGH6
GenBank Number:

X13461

Species: Homo sapiens, human
Depositors: EE Strehler
Vector:
Construct size (kb): 5.400000095367432
Insert:
DNA: genomic
Insert lengths(kb): 2.733999967575073
Tissue: leukocyte
Gene product: calmodulin-like CALML3
Insert Size (kb): 2.734
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.3, 1.15; PvuII--2.4, 1.1, 0.95, 0.75, 0.35; SacI--2.7 (doublet); SacI/BglI--1.4, 1.15, 0.9, 0.85, 0.65, 0.4; SmaI--3.7, 1.2, 0.75; SmaI/SacI--2.7, 1.2, 0.9, 0.75.
The insert contains the following restriction sites (nt from the 5 end): PstI--106, 752, 1741, 1905, 1998, 2208; PvuII--162, 1077, 2144; SmaI--863, 1569; NotI--953; EcoRI--1099.
Contains a complete open reading frame of 444 nucleotides, with 5 and 3 flanking sequences.
A 0.7 kb SmaI fragment containing the coding region (nt 863 -1569) detects a 2.3 kb mRNA in human erythroleukemia K562 cells. A 1.2 kb SmaI/SacI fragment from the 3 region (nt 1570 - 2738) detects a 1.5 kb mRNA in SV40-transformed human fibroblasts.
The intact insert can be separated from the vector by a SacI/SmaI double digest.
References:

Koller M, Strehler EE. Characterization of an intronless human calmodulin-like pseudogene. FEBS Lett. 239: 121-128, 1988. PubMed: 3181418

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phgH6 [hGH6]

  • 货号: TS147921
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: phgH6 hGH6
商品货号: TS147921
Designations: phgH6 hGH6
GenBank Number:

X13461

Species: Homo sapiens, human
Depositors: EE Strehler
Vector:
Construct size (kb): 5.400000095367432
Insert:
DNA: genomic
Insert lengths(kb): 2.733999967575073
Tissue: leukocyte
Gene product: calmodulin-like CALML3
Insert Size (kb): 2.734
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.3, 1.15; PvuII--2.4, 1.1, 0.95, 0.75, 0.35; SacI--2.7 (doublet); SacI/BglI--1.4, 1.15, 0.9, 0.85, 0.65, 0.4; SmaI--3.7, 1.2, 0.75; SmaI/SacI--2.7, 1.2, 0.9, 0.75.
The insert contains the following restriction sites (nt from the 5 end): PstI--106, 752, 1741, 1905, 1998, 2208; PvuII--162, 1077, 2144; SmaI--863, 1569; NotI--953; EcoRI--1099.
Contains a complete open reading frame of 444 nucleotides, with 5 and 3 flanking sequences.
A 0.7 kb SmaI fragment containing the coding region (nt 863 -1569) detects a 2.3 kb mRNA in human erythroleukemia K562 cells. A 1.2 kb SmaI/SacI fragment from the 3 region (nt 1570 - 2738) detects a 1.5 kb mRNA in SV40-transformed human fibroblasts.
The intact insert can be separated from the vector by a SacI/SmaI double digest.
References:

Koller M, Strehler EE. Characterization of an intronless human calmodulin-like pseudogene. FEBS Lett. 239: 121-128, 1988. PubMed: 3181418

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