| 产品名称： | Blakeslea trispora Thaxter |
|---|---|
| 商品货号： | TS147944 |
| Strain Designations： | NRRL 2457 CBS 131.59, IMI 195169, QM 6309 |
| Application： | classroom demonstration produces beta-carotene |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Mating Type： | minus |
| Medium： | ATCC® Medium 340: Rabbit food agar ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28/26S ribosomal RNA gene, partial sequence CATTAAATAAATAAGAAGTAATTAGGCTCGTCCTGTTACTATCTATTTTACTGTGAACTGTTTTATTTTRCAGCGTTTGAGGGATGTTCTTGTGCTATATGGGTAGGCATAAGAAATGTTAACCGAGCTGTAATCAGGCTTAGGCTTGGTACCCTGTTTATATACTTTCAATTGATCAGATTTTAAAATGTAACATAGAGTAAAATCTATAAAACAACTTTTAACAACGGATCTCTTGGCTTTTGCATCGATGAAGAACGTAGCAAATTGCGATAACTAGTGTGAATTGCAAATTCAGTGAATCATCGAGTCTTTGAACGCATCTTGCGCTCATTGGTATTCCAGTGAGCACGCCTGTTTCAGTATCAAAAACAACCCTCATTCAAATTTTTTTTTGAATGGTCATGAAGGAAGCTAACTTGTTGGCGACCTTTTAAATTGAGTAAGGCCTGAATTTGTTTATCTAGCCTGAACTTTTTTTTAATATAAAGGAAAGCTCTTGCGACTTGAACTTTGTTGGGGCCTCCCAAATAAAGCTT D1D2 region of the 28/26S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAAATAACAATGATTTCCCTAGTAACGGCGAGTGAACAGGAAAGAGCTCAAAGTTGGAACCTGTTTGGTTTAGCCAAACCGGATTGTAAACTGTAGAAGTGTTTTCCTGGCACGCCTAACGAAAAAGTCCATTGGAACATGGTGACATAGAGGGTGAGAGCCCCGTATTTTGTTAGAGCTGTTGTCTTTTGTGATACACTTTCAAAGAGTCAGGTTGTTTGGGAATGCAGCCTAAATTGGGTGGTAAATCTCACCTAAAGCTAAATATTGGCAAAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGAACTTTGAAAAGAGAGTTAAACAGTATGTGAAATTGTTAAAAGGGAACCGTTTGGAGCCAGACTGGCTTGATTGTAATCAATCTAGATYTCGATCTGGATGCACTTGCAGTCTATGCCTGCCAACGACAATTCATTCTGGAGGAAAAAACTAGGAGGAATGTGGCCCCTCGGGGTGTTATAGACTTTTAGAAAATACTCTGGTTTGGATTGAGGAACGCAGCAGATGCTATTTGGCGAAGGTGCTGAGTGCTTTTAGTTTTATTGGTTAGAATTTCTGCTTCGGGTGGTGCTAATCAATACGATTGGAACTCGCTTAGTTCACTTTTTATCTGCTTAGGTTGTTGGCTTAATGACTCTAAATGA |
| Name of Depositor： | NRRL |
| Chain of Custody： | ATCC |
| References： | Anderson RF. Method for the production of carotenes. US Patent 2,890,989 dated Jun 16 1959 Hesseltine CW, Anderson RF. Method of making carotenes and related substances by mixed culture fermentation. US Patent 2,865,814 dated Dec 23 1958 ODonnell KL. Zygosporangial types of several Mucoralean families In: Lower fungi in the laboratory. Athens, GA Univ., Georgia. pp. 143-144, 1978. Ciegler A, Arnold M, Anderson RF. Microbiological production of carotenoids. IV. Effect of various grains on production of beta-carotene by mated strains of Blakeslea trispora. Appl Microbiol 7: 94-101, 1959. PubMed: 13637891 Kim SW, et al. Enhanced synthesis of trisporic acid and beta-carotene production in Blakeslea trispora by addition of a non-ionic surfactant, Span 20. J. Ferment. Bioeng. 84: 330-332, 1997. Anderson RE, et al. Microbiological production of beta-carotene in shaken flasks. Agric. Food Chem. 6: 543-545, 1958. Hesseltine CW, Anderson RF. Microbiological production of carotenoids. I. Zygospores and carotene produced by intraspecific and interspecific crosses of Choanephoraceae in liquid media. Mycologia 49: 449-452, 1957. |
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| 产品名称： | Blakeslea trispora Thaxter |
|---|---|
| 商品货号： | TS147944 |
| Strain Designations： | NRRL 2457 CBS 131.59, IMI 195169, QM 6309 |
| Application： | classroom demonstration produces beta-carotene |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Mating Type： | minus |
| Medium： | ATCC® Medium 340: Rabbit food agar ATCC® Medium 336: Potato dextrose agar (PDA) |
| Growth Conditions： | Temperature: 24°C to 26°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28/26S ribosomal RNA gene, partial sequence CATTAAATAAATAAGAAGTAATTAGGCTCGTCCTGTTACTATCTATTTTACTGTGAACTGTTTTATTTTRCAGCGTTTGAGGGATGTTCTTGTGCTATATGGGTAGGCATAAGAAATGTTAACCGAGCTGTAATCAGGCTTAGGCTTGGTACCCTGTTTATATACTTTCAATTGATCAGATTTTAAAATGTAACATAGAGTAAAATCTATAAAACAACTTTTAACAACGGATCTCTTGGCTTTTGCATCGATGAAGAACGTAGCAAATTGCGATAACTAGTGTGAATTGCAAATTCAGTGAATCATCGAGTCTTTGAACGCATCTTGCGCTCATTGGTATTCCAGTGAGCACGCCTGTTTCAGTATCAAAAACAACCCTCATTCAAATTTTTTTTTGAATGGTCATGAAGGAAGCTAACTTGTTGGCGACCTTTTAAATTGAGTAAGGCCTGAATTTGTTTATCTAGCCTGAACTTTTTTTTAATATAAAGGAAAGCTCTTGCGACTTGAACTTTGTTGGGGCCTCCCAAATAAAGCTT D1D2 region of the 28/26S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAAATAACAATGATTTCCCTAGTAACGGCGAGTGAACAGGAAAGAGCTCAAAGTTGGAACCTGTTTGGTTTAGCCAAACCGGATTGTAAACTGTAGAAGTGTTTTCCTGGCACGCCTAACGAAAAAGTCCATTGGAACATGGTGACATAGAGGGTGAGAGCCCCGTATTTTGTTAGAGCTGTTGTCTTTTGTGATACACTTTCAAAGAGTCAGGTTGTTTGGGAATGCAGCCTAAATTGGGTGGTAAATCTCACCTAAAGCTAAATATTGGCAAAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGAACTTTGAAAAGAGAGTTAAACAGTATGTGAAATTGTTAAAAGGGAACCGTTTGGAGCCAGACTGGCTTGATTGTAATCAATCTAGATYTCGATCTGGATGCACTTGCAGTCTATGCCTGCCAACGACAATTCATTCTGGAGGAAAAAACTAGGAGGAATGTGGCCCCTCGGGGTGTTATAGACTTTTAGAAAATACTCTGGTTTGGATTGAGGAACGCAGCAGATGCTATTTGGCGAAGGTGCTGAGTGCTTTTAGTTTTATTGGTTAGAATTTCTGCTTCGGGTGGTGCTAATCAATACGATTGGAACTCGCTTAGTTCACTTTTTATCTGCTTAGGTTGTTGGCTTAATGACTCTAAATGA |
| Name of Depositor： | NRRL |
| Chain of Custody： | ATCC |
| References： | Anderson RF. Method for the production of carotenes. US Patent 2,890,989 dated Jun 16 1959 Hesseltine CW, Anderson RF. Method of making carotenes and related substances by mixed culture fermentation. US Patent 2,865,814 dated Dec 23 1958 ODonnell KL. Zygosporangial types of several Mucoralean families In: Lower fungi in the laboratory. Athens, GA Univ., Georgia. pp. 143-144, 1978. Ciegler A, Arnold M, Anderson RF. Microbiological production of carotenoids. IV. Effect of various grains on production of beta-carotene by mated strains of Blakeslea trispora. Appl Microbiol 7: 94-101, 1959. PubMed: 13637891 Kim SW, et al. Enhanced synthesis of trisporic acid and beta-carotene production in Blakeslea trispora by addition of a non-ionic surfactant, Span 20. J. Ferment. Bioeng. 84: 330-332, 1997. Anderson RE, et al. Microbiological production of beta-carotene in shaken flasks. Agric. Food Chem. 6: 543-545, 1958. Hesseltine CW, Anderson RF. Microbiological production of carotenoids. I. Zygospores and carotene produced by intraspecific and interspecific crosses of Choanephoraceae in liquid media. Mycologia 49: 449-452, 1957. |
