| 产品名称： | pPGBC9 |
|---|---|
| 商品货号： | TS148055 |
| Designations： | pPGBC9 |
| Species： | Homo sapiens, human |
| Depositors： | P van den Elsen |
| Applications： | Codominant segregation was observed in 4 unrelated families (49 individuals). Plasmid pPGBC9 was cut with BamHI and the fragments of 0.5 kb and 0.6 kb were isolated, pooled and used as the probe for the MspI and TaqI RFLPs. |
| Vector： | Construct size (kb): 3.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.699999988079071 Tissue: T4+,T8- T-cell clone Gene product: antigen CD3D, delta polypeptide (TiT3 complex) T3D Alleles: B2, B2, B1, B1, , , , , , , , A1, A2, , , , , , , , A1, A2 |
| Insert Size (kb)： | 0.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.9; SalI--3.9; PstI--2.5, 1.3; BamHI--2.9, 0.6, 0.5. TaqI and MspI RFLP alleles are in strong linkage disequilibrium. Codominant segregation was observed in 4 unrelated families (49 individuals). The insert contains ATG at 97, TGA at 610, and 89 bp of 3 untranslated sequence including AATAA followed by poly(A) 17 bp downstream. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Plasmid pPGBC9 was cut with BamHI and the fragments of 0.5 kb and 0.6 kb were isolated, pooled and used as the probe for the MspI and TaqI RFLPs. Enzyme(s) not detecting polymorphism: RsaI, BstEII, EcoRV, BamHI, HindIII, BglII, PvuII, BglI and PstI in at least 6 unrelated individuals. |
| References： | Malhotra U, Concannon P. Human T-cell receptor CD3-delta (CD3D) / MspI DNA polymorphism. Nucleic Acids Res. 17: 2373, 1989. PubMed: 2468134 van den Elsen P, et al. Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex. Nature 312: 413-418, 1984. PubMed: 6095101 Charmley P, et al. A primary linkage map of the human chromosome 11q22-23 region. Genomics 6: 316-323, 1990. PubMed: 2307473 Barbara Weiffenbach, personal communication |
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| 产品名称： | pPGBC9 |
|---|---|
| 商品货号： | TS148055 |
| Designations： | pPGBC9 |
| Species： | Homo sapiens, human |
| Depositors： | P van den Elsen |
| Applications： | Codominant segregation was observed in 4 unrelated families (49 individuals). Plasmid pPGBC9 was cut with BamHI and the fragments of 0.5 kb and 0.6 kb were isolated, pooled and used as the probe for the MspI and TaqI RFLPs. |
| Vector： | Construct size (kb): 3.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.699999988079071 Tissue: T4+,T8- T-cell clone Gene product: antigen CD3D, delta polypeptide (TiT3 complex) T3D Alleles: B2, B2, B1, B1, , , , , , , , A1, A2, , , , , , , , A1, A2 |
| Insert Size (kb)： | 0.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--3.9; SalI--3.9; PstI--2.5, 1.3; BamHI--2.9, 0.6, 0.5. TaqI and MspI RFLP alleles are in strong linkage disequilibrium. Codominant segregation was observed in 4 unrelated families (49 individuals). The insert contains ATG at 97, TGA at 610, and 89 bp of 3 untranslated sequence including AATAA followed by poly(A) 17 bp downstream. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Plasmid pPGBC9 was cut with BamHI and the fragments of 0.5 kb and 0.6 kb were isolated, pooled and used as the probe for the MspI and TaqI RFLPs. Enzyme(s) not detecting polymorphism: RsaI, BstEII, EcoRV, BamHI, HindIII, BglII, PvuII, BglI and PstI in at least 6 unrelated individuals. |
| References： | Malhotra U, Concannon P. Human T-cell receptor CD3-delta (CD3D) / MspI DNA polymorphism. Nucleic Acids Res. 17: 2373, 1989. PubMed: 2468134 van den Elsen P, et al. Isolation of cDNA clones encoding the 20K T3 glycoprotein of human T-cell receptor complex. Nature 312: 413-418, 1984. PubMed: 6095101 Charmley P, et al. A primary linkage map of the human chromosome 11q22-23 region. Genomics 6: 316-323, 1990. PubMed: 2307473 Barbara Weiffenbach, personal communication |
