| 产品名称： | KBP5 |
|---|---|
| 商品货号： | TS149578 |
| Designations： | KBP5 |
| GenBank Number： | U07000 |
| Species： | Homo sapiens, human |
| Depositors： | S Clifton, BA Roe |
| Vector： | Construct size (kb): 48.40000152587891 DESCRIPTION OF VECTOR COMPONENT: Name of vector: svPHEP Intact vector size: 9.201 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI ClaI Polylinker sites: HindIII ClaI EcoRI BamHI Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR replicon: pMB1 Cross references: DNA Seq. Acc.: L19900 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: BCR Other gene symbols: BCR1 Genomic copy number: unique Gene name: breakpoint cluster region Contains complete coding sequence?: N Chromosome: 22; Localization: 22 q11 Tissue: chronic myelogenous leukemia K-562 cell line Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 39.199 Cross references: DNA Seq. Acc.: U07000 Nucleotides 1-39199 of the insert correspond to nucleotides 85435-124633 of U07000. Insert lengths(kb): 39.19900131225586 Tissue: chronic myelogenous leukemia K-562 cell line Gene product: breakpoint cluster region BCR Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 39.199 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 12.5, 10.5, 7.0, 1.45, 0.52; BglII--13.5, 9.8, 7.0, 6.2, 4.8, 2.7, 2.6, 1.75, 0.92, 0.54; EcoRI--20.0, 17.0, 6.6, 2.75, 1.2, 1.1, 0.6; SstI--7.5, 6.4, 4.4, 3.8, 3.5 (doublet), 3.35, 2.15, 2.05, 1.7, 1.45, 1.3, 0.62, 0.4; XbaI--18.0 (doublet), 10.5, 2.35; SalI--33.0, 16.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert begins in intron 3 and continues into exon 15. This region includes an open reading frame (96845-97681) that appears to be expressed. |
| References： | Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859 Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185 Bruce A Roe, personal communication |
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| 产品名称： | KBP5 |
|---|---|
| 商品货号： | TS149578 |
| Designations： | KBP5 |
| GenBank Number： | U07000 |
| Species： | Homo sapiens, human |
| Depositors： | S Clifton, BA Roe |
| Vector： | Construct size (kb): 48.40000152587891 DESCRIPTION OF VECTOR COMPONENT: Name of vector: svPHEP Intact vector size: 9.201 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI ClaI Polylinker sites: HindIII ClaI EcoRI BamHI Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR replicon: pMB1 Cross references: DNA Seq. Acc.: L19900 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: BCR Other gene symbols: BCR1 Genomic copy number: unique Gene name: breakpoint cluster region Contains complete coding sequence?: N Chromosome: 22; Localization: 22 q11 Tissue: chronic myelogenous leukemia K-562 cell line Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 39.199 Cross references: DNA Seq. Acc.: U07000 Nucleotides 1-39199 of the insert correspond to nucleotides 85435-124633 of U07000. Insert lengths(kb): 39.19900131225586 Tissue: chronic myelogenous leukemia K-562 cell line Gene product: breakpoint cluster region BCR Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 39.199 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 12.5, 10.5, 7.0, 1.45, 0.52; BglII--13.5, 9.8, 7.0, 6.2, 4.8, 2.7, 2.6, 1.75, 0.92, 0.54; EcoRI--20.0, 17.0, 6.6, 2.75, 1.2, 1.1, 0.6; SstI--7.5, 6.4, 4.4, 3.8, 3.5 (doublet), 3.35, 2.15, 2.05, 1.7, 1.45, 1.3, 0.62, 0.4; XbaI--18.0 (doublet), 10.5, 2.35; SalI--33.0, 16.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert begins in intron 3 and continues into exon 15. This region includes an open reading frame (96845-97681) that appears to be expressed. |
| References： | Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859 Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185 Bruce A Roe, personal communication |
