AR4c-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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AR4c
AR4c
规格:
货期:
编号:TS149870
品牌:Testobio
产品名称: AR4c
商品货号: TS149870
Designations: AR4c
GenBank Number:

X83543

Species: Homo sapiens, human
Depositors: MV Schiaffino
Applications:
contains sequence apical protein, Xenopus laevis-like
Vector:
Construct size (kb): 6.699999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: APXL
Gene name: apical protein, Xenopus laevis-like
Contains complete coding sequence?: N
Tissue: retina, adult
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.647
Cross references: DNA Seq. Acc.: X83543
Nucleotides 1-3637 of the insert correspond to
nucleotides 3808-7445 of X83543.
Insert lengths(kb): 3.647000074386597
Tissue: retina, adult
Gene product: apical protein, Xenopus laevis-like APXL
Target Gene: apical protein, Xenopus laevis-like
Insert Size (kb): 3.647
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 3.0; BamHI--6.3, 0.4; HindIII-- 6.0, 0.7.
The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.35; BglI--0.05, 0.15, 0.55, 2.85, 2.9; HindIII--3.05; NcoI--2.15; SacI--0.65, 1.0, 1.25; SmaI--0.85; PstI--0.6, 0.7; PvuII--1.15, 3.05, 3.35, 3.5; XhoI--3.3.
The insert contains a portion of the coding sequence and 2509 bp of 3 untranslated sequence, overlapping AR24 (ATCC 99512) at the 5 end.
The coding region of GenBank X83543 corresponds to nt 91-4941.
References:

Schiaffino MV, et al. Cloning of a human homologue of the Xenopus laevis APX gene from the ocular albinism type 1 critical region. Hum. Mol. Genet. 4: 373-382, 1995. PubMed: 7795590

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AR4c

  • 货号: TS149870
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: AR4c
商品货号: TS149870
Designations: AR4c
GenBank Number:

X83543

Species: Homo sapiens, human
Depositors: MV Schiaffino
Applications:
contains sequence apical protein, Xenopus laevis-like
Vector:
Construct size (kb): 6.699999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: APXL
Gene name: apical protein, Xenopus laevis-like
Contains complete coding sequence?: N
Tissue: retina, adult
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.647
Cross references: DNA Seq. Acc.: X83543
Nucleotides 1-3637 of the insert correspond to
nucleotides 3808-7445 of X83543.
Insert lengths(kb): 3.647000074386597
Tissue: retina, adult
Gene product: apical protein, Xenopus laevis-like APXL
Target Gene: apical protein, Xenopus laevis-like
Insert Size (kb): 3.647
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 3.0; BamHI--6.3, 0.4; HindIII-- 6.0, 0.7.
The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.35; BglI--0.05, 0.15, 0.55, 2.85, 2.9; HindIII--3.05; NcoI--2.15; SacI--0.65, 1.0, 1.25; SmaI--0.85; PstI--0.6, 0.7; PvuII--1.15, 3.05, 3.35, 3.5; XhoI--3.3.
The insert contains a portion of the coding sequence and 2509 bp of 3 untranslated sequence, overlapping AR24 (ATCC 99512) at the 5 end.
The coding region of GenBank X83543 corresponds to nt 91-4941.
References:

Schiaffino MV, et al. Cloning of a human homologue of the Xenopus laevis APX gene from the ocular albinism type 1 critical region. Hum. Mol. Genet. 4: 373-382, 1995. PubMed: 7795590

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