| 产品名称： | plambdaP2 pP2 |
|---|---|
| 商品货号： | TS150003 |
| Designations： | plambdaP2 pP2 |
| GenBank Number： | J00290 |
| Species： | Homo sapiens, human |
| Depositors： | SN Cohen |
| Applications： | Enzyme(s) not detecting polymorphism: BglII, PstI, BamHI, AvaII, BglI, MspI. Tested against 20 unrelated individuals. |
| Vector： | Construct size (kb): 6.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 1.899999976158142 Tissue: liver Gene product: proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin)(adrenocorticotropic hormone (ACTH)) POMC Alleles: A1, A2, B1, B2 |
| Insert Size (kb)： | 1.900 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.3; HindIII--6.3; PstI--4.4, 1.9. The insert includes 5 flanking sequences and exon 1. SstI polymorphism is about 10 kb 5 to exon 1 of POMC. Shows Mendelian segregation with codominance. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Enzyme(s) not detecting polymorphism: BglII, PstI, BamHI, AvaII, BglI, MspI. Tested against 20 unrelated individuals. |
| References： | Feder J, et al. A DNA polymorphism in close physical linkage with the proopimelanocortin gene. Am. J. Hum. Genet. 35: 1090-1096, 1983. PubMed: 6316780 Feder J, et al. DNA restriction fragment analysis of the proopiomelanocortin gene in schizophrenia and bipolar disorders. Am. J. Hum. Genet. 37: 286-294, 1985. PubMed: 2984925 Cochet M, et al. Characterization of the structural gene and putative 5-regulatory sequences for human proopiomelanocortin. Nature 297: 335-339, 1982. PubMed: 6927850 Barbara Weiffenbach, personal communication |
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| 产品名称： | plambdaP2 pP2 |
|---|---|
| 商品货号： | TS150003 |
| Designations： | plambdaP2 pP2 |
| GenBank Number： | J00290 |
| Species： | Homo sapiens, human |
| Depositors： | SN Cohen |
| Applications： | Enzyme(s) not detecting polymorphism: BglII, PstI, BamHI, AvaII, BglI, MspI. Tested against 20 unrelated individuals. |
| Vector： | Construct size (kb): 6.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 1.899999976158142 Tissue: liver Gene product: proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin)(adrenocorticotropic hormone (ACTH)) POMC Alleles: A1, A2, B1, B2 |
| Insert Size (kb)： | 1.900 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.3; HindIII--6.3; PstI--4.4, 1.9. The insert includes 5 flanking sequences and exon 1. SstI polymorphism is about 10 kb 5 to exon 1 of POMC. Shows Mendelian segregation with codominance. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Enzyme(s) not detecting polymorphism: BglII, PstI, BamHI, AvaII, BglI, MspI. Tested against 20 unrelated individuals. |
| References： | Feder J, et al. A DNA polymorphism in close physical linkage with the proopimelanocortin gene. Am. J. Hum. Genet. 35: 1090-1096, 1983. PubMed: 6316780 Feder J, et al. DNA restriction fragment analysis of the proopiomelanocortin gene in schizophrenia and bipolar disorders. Am. J. Hum. Genet. 37: 286-294, 1985. PubMed: 2984925 Cochet M, et al. Characterization of the structural gene and putative 5-regulatory sequences for human proopiomelanocortin. Nature 297: 335-339, 1982. PubMed: 6927850 Barbara Weiffenbach, personal communication |
