| 产品名称： | p304 |
|---|---|
| 商品货号： | TS150127 |
| Designations： | p304 |
| GenBank Number： | J00127 |
| Species： | Homo sapiens, human |
| Depositors： | ST Lord |
| Applications： | The depositor notes that the fibrinogen clones (TS150127-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells. |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: liver Gene product: fibrinogen, A alpha polypeptide FGA Alleles: A1, A2 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.2, 1.9, 0.4; XhoI--4.8; EcoRI/XhoI--2.1, 1.1, 0.98; PstI--3.3, 1.1; BglI--3.2, 1.2; EcoRI/HindIII--2.3, 1.9; NcoI--2.5, 1.9. Insert encodes fibrinogen A alpha amino acids 1-625 with an initiating ATG in front of amino acid 1. The vector is an expression vector. The depositor notes that the fibrinogen clones (TS150127-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells. The clones do not encode the signal peptide necessary for assembly and secretion and one is known to lack a single nucleotide in the coding region. The ability of this probe to detect the TaqI polymorphism was verified by J. Murray, University of Iowa. |
| References： | Chung DW, et al. Characterization of complementary deoxyribonucleic acid and genomic deoxyribonucleic acid for the beta-chain of human fibronogen. Biochemistry 22: 3244-3250, 1983. PubMed: 6688356 Lord ST. Expression of a cloned human fibrinogen cDNA in Escherichia coli: synthesis of an A alpha polypeptide. DNA 4: 33-38, 1985. PubMed: 3882374 Susan T Lord, personal communication |
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| 产品名称： | p304 |
|---|---|
| 商品货号： | TS150127 |
| Designations： | p304 |
| GenBank Number： | J00127 |
| Species： | Homo sapiens, human |
| Depositors： | ST Lord |
| Applications： | The depositor notes that the fibrinogen clones (TS150127-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells. |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: liver Gene product: fibrinogen, A alpha polypeptide FGA Alleles: A1, A2 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.2, 1.9, 0.4; XhoI--4.8; EcoRI/XhoI--2.1, 1.1, 0.98; PstI--3.3, 1.1; BglI--3.2, 1.2; EcoRI/HindIII--2.3, 1.9; NcoI--2.5, 1.9. Insert encodes fibrinogen A alpha amino acids 1-625 with an initiating ATG in front of amino acid 1. The vector is an expression vector. The depositor notes that the fibrinogen clones (TS150127-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells. The clones do not encode the signal peptide necessary for assembly and secretion and one is known to lack a single nucleotide in the coding region. The ability of this probe to detect the TaqI polymorphism was verified by J. Murray, University of Iowa. |
| References： | Chung DW, et al. Characterization of complementary deoxyribonucleic acid and genomic deoxyribonucleic acid for the beta-chain of human fibronogen. Biochemistry 22: 3244-3250, 1983. PubMed: 6688356 Lord ST. Expression of a cloned human fibrinogen cDNA in Escherichia coli: synthesis of an A alpha polypeptide. DNA 4: 33-38, 1985. PubMed: 3882374 Susan T Lord, personal communication |
