| 产品名称： | pRK2013 |
|---|---|
| 商品货号： | TS150205 |
| Designations： | pRK2013 |
| Depositors： | EW Nester |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Size (kb): 48.0
Vector: pRK2013 (plasmid)
Construction: pPRK212.2, pDF1
Marker(s): kanR
Construct size (kb): 48.0
Features:
marker(s): kanR
replicon: RK2 |
| Applications： | mobilization helper plasmid |
| Comments： | This is a helper plasmid for mobilization of non-self-transmissible plasmids. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37°C |
| References： | Knauf VC, Nester EW. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium ti plasmid. Plasmid 8: 45-54, 1982. PubMed: 6292969 Figurski DH, Helinski DR. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76: 1648-1652, 1979. PubMed: 377280 |
| Shipped： | frozen |
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| 产品名称： | pRK2013 |
|---|---|
| 商品货号： | TS150205 |
| Designations： | pRK2013 |
| Depositors： | EW Nester |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Size (kb): 48.0
Vector: pRK2013 (plasmid)
Construction: pPRK212.2, pDF1
Marker(s): kanR
Construct size (kb): 48.0
Features:
marker(s): kanR
replicon: RK2 |
| Applications： | mobilization helper plasmid |
| Comments： | This is a helper plasmid for mobilization of non-self-transmissible plasmids. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37°C |
| References： | Knauf VC, Nester EW. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium ti plasmid. Plasmid 8: 45-54, 1982. PubMed: 6292969 Figurski DH, Helinski DR. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76: 1648-1652, 1979. PubMed: 377280 |
| Shipped： | frozen |
