| 产品名称： | hp2alpha hp2-alpha |
|---|---|
| 商品货号： | TS150298 |
| Designations： | hp2alpha hp2-alpha |
| GenBank Number： | X00606 |
| Species： | Homo sapiens, human |
| Applications： | A small HindIII/PstI linker was used in the process of inserting the PstI/BamHI fragment into the HindIII and BamHI sites of pAT153. |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.5 Tissue: adult Gene product: haptoglobin HP Alleles: B1, B2, A1, A1, A2, A2, C1, C2 |
| Insert Size (kb)： | 0.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--3.25, 0.6; BamHI--3.8; PstI--2.8, 0.71; HindIII--3.8; EcoRI--3.8. The insert covers the entire alpha chain coding region and leader. There are typically two copies of the gene per haploid genome. HindIII detects a 1.7 kb length polymorphism which is also detected by BamHI with fragment sizes of 7.5 kb and 9.2 kb. The HindIII polymorphism is also detected by AvaII with fragment sizes of 4.4 kb (freq = 0.65) and 2.7 (freq = 0.35). The AvaII RFLP also appears to be in linkage equilibrium with a TaqI RFLP at the CETP locus. There is a polymorphic EcoRI site about 4 kb 3 to Hp2 which reduces the allele size to 10.1 (indistinguishable from the Hp1 allele). For the XbaI polymorphism, the 5.8 kb constant band is from the HPR sequences, the 4.6 kb band is from the Hp2 or Hp1F alleles, and the 4.0 kb band is from the Hp1S allele. For the HindIII polymorphism, the 3.4 kb constant band is from the HPR sequences, the 4.9 kb band is from the Hp2 allele, and the 3.2 kb band is from the Hp1S or Hp1F alleles. For the EcoRI polymorphism, the 8.3 kb constant band is from the HPR sequences, the 11.8 kb band is from the Hp2 allele, and the 10.1 kb band is from the Hp1S or Hp1F alleles. A small HindIII/PstI linker was used in the process of inserting the PstI/BamHI fragment into the HindIII and BamHI sites of pAT153. |
| References： | Bashir A, et al. An AvaII polymorphism in the haptoglobin alpha gene (HPA). Nucleic Acids Res. 17: 4906, 1989. PubMed: 2568614 Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217 Maeda N, et al. Duplication within the haptoglobin HP2 gene. Nature 309: 131-135, 1984. PubMed: 6325933 Nobuyo Maeda, personal communication |
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| 产品名称： | hp2alpha hp2-alpha |
|---|---|
| 商品货号： | TS150298 |
| Designations： | hp2alpha hp2-alpha |
| GenBank Number： | X00606 |
| Species： | Homo sapiens, human |
| Applications： | A small HindIII/PstI linker was used in the process of inserting the PstI/BamHI fragment into the HindIII and BamHI sites of pAT153. |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.5 Tissue: adult Gene product: haptoglobin HP Alleles: B1, B2, A1, A1, A2, A2, C1, C2 |
| Insert Size (kb)： | 0.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--3.25, 0.6; BamHI--3.8; PstI--2.8, 0.71; HindIII--3.8; EcoRI--3.8. The insert covers the entire alpha chain coding region and leader. There are typically two copies of the gene per haploid genome. HindIII detects a 1.7 kb length polymorphism which is also detected by BamHI with fragment sizes of 7.5 kb and 9.2 kb. The HindIII polymorphism is also detected by AvaII with fragment sizes of 4.4 kb (freq = 0.65) and 2.7 (freq = 0.35). The AvaII RFLP also appears to be in linkage equilibrium with a TaqI RFLP at the CETP locus. There is a polymorphic EcoRI site about 4 kb 3 to Hp2 which reduces the allele size to 10.1 (indistinguishable from the Hp1 allele). For the XbaI polymorphism, the 5.8 kb constant band is from the HPR sequences, the 4.6 kb band is from the Hp2 or Hp1F alleles, and the 4.0 kb band is from the Hp1S allele. For the HindIII polymorphism, the 3.4 kb constant band is from the HPR sequences, the 4.9 kb band is from the Hp2 allele, and the 3.2 kb band is from the Hp1S or Hp1F alleles. For the EcoRI polymorphism, the 8.3 kb constant band is from the HPR sequences, the 11.8 kb band is from the Hp2 allele, and the 10.1 kb band is from the Hp1S or Hp1F alleles. A small HindIII/PstI linker was used in the process of inserting the PstI/BamHI fragment into the HindIII and BamHI sites of pAT153. |
| References： | Bashir A, et al. An AvaII polymorphism in the haptoglobin alpha gene (HPA). Nucleic Acids Res. 17: 4906, 1989. PubMed: 2568614 Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217 Maeda N, et al. Duplication within the haptoglobin HP2 gene. Nature 309: 131-135, 1984. PubMed: 6325933 Nobuyo Maeda, personal communication |
