lambdaepsilon10EZZ-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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lambdaepsilon10EZZ
lambdaepsilon10EZZ
规格:
货期:
编号:TS152241
品牌:Testobio
产品名称: lambdaepsilon10EZZ
商品货号: TS152241
Designations: lambdaepsilon10EZZ
Species: Homo sapiens, human
Depositors: M Lefranc, TH Rabbitts
Vector:
Construct size (kb): 44.0
Insert:
DNA: genomic
Insert lengths(kb): 14.39999961853027
Tissue: B lymphocyte (EBV transformed)
Gene product: immunoglobulin epsilon IGHE
Insert Size (kb): 14.400
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried bacteria-free lysate
Comments:
Restriction digests of the clone give the following sizes (kb): XbaI--20.0, 11.0, 9.2, 3.8; SacI--20.0, 9.2, 3.8, 2.9, 2.3, 1.8, 1.2, 1.0, 0.65, 0.58, 0.52; XhoI--20.0, 14.0, 9.2;
SmaI--20.0, 5.8, 3.7, 3.5, 1.25, 0.90, 0.84, 0.76; PvuI--20.0, 12.0, 8.2, 5.8; HindIII--23.0, 9.2, 3.9, 0.90, 0.65.
The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--3.9, 4.8, 5.5; BamHI--7.2, 10.
Cloned from DNA isolated from individual II-4 of the TOU family.
A 3.9 kb HindIII fragment from the 5 end of this insert, pH3.9EZZ, has been deposited independently as ATCC 61230, 61231. The 5 HindIII site is vector-derived.
A 2.8 kb BamHI fragment contains the exons encoding the epsilon constant region.
References:

Keyeux G, et al. A multigene deletion in the human IGH constant region locus involves highly homologous hot spots of recombination. Genomics 5: 431-441, 1989. PubMed: 2613231

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lambdaepsilon10EZZ

  • 货号: TS152241
  • 好评
有货
  • 品牌 : TESTOBIO
产品名称: lambdaepsilon10EZZ
商品货号: TS152241
Designations: lambdaepsilon10EZZ
Species: Homo sapiens, human
Depositors: M Lefranc, TH Rabbitts
Vector:
Construct size (kb): 44.0
Insert:
DNA: genomic
Insert lengths(kb): 14.39999961853027
Tissue: B lymphocyte (EBV transformed)
Gene product: immunoglobulin epsilon IGHE
Insert Size (kb): 14.400
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried bacteria-free lysate
Comments:
Restriction digests of the clone give the following sizes (kb): XbaI--20.0, 11.0, 9.2, 3.8; SacI--20.0, 9.2, 3.8, 2.9, 2.3, 1.8, 1.2, 1.0, 0.65, 0.58, 0.52; XhoI--20.0, 14.0, 9.2;
SmaI--20.0, 5.8, 3.7, 3.5, 1.25, 0.90, 0.84, 0.76; PvuI--20.0, 12.0, 8.2, 5.8; HindIII--23.0, 9.2, 3.9, 0.90, 0.65.
The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--3.9, 4.8, 5.5; BamHI--7.2, 10.
Cloned from DNA isolated from individual II-4 of the TOU family.
A 3.9 kb HindIII fragment from the 5 end of this insert, pH3.9EZZ, has been deposited independently as ATCC 61230, 61231. The 5 HindIII site is vector-derived.
A 2.8 kb BamHI fragment contains the exons encoding the epsilon constant region.
References:

Keyeux G, et al. A multigene deletion in the human IGH constant region locus involves highly homologous hot spots of recombination. Genomics 5: 431-441, 1989. PubMed: 2613231

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