| 产品名称： | Tetramitis thorntoni (Singh) Brown and De Jonckheere |
|---|---|
| 商品货号： | TS163504 |
| Deposited As： | Naegleria thorntoni (Singh) Page |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | farmyard soil, England, 1950 (?) |
| Product Format： | freeze-dried |
| Type Strain： | no |
| Comments： | Zymodeme Th-1 Maximum temperature tolerance 37C Circular ribosomal RNA genes Transfer of species back to Didasculus based upon SSUrDNA sequence review |
| Medium： | ATCC® Medium 997: Fresh water ameba medium |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Escherichia coli ATCC 11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Subcultivation： | Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Name of Depositor： | W Balamuth |
| Chain of Custody： | ATCC < |
| References： | Clark CG, Cross GA. Circular ribosomal RNA genes are a general feature of schizopyrenid amoebae. J. Protozool. 35: 326-329, 1988. PubMed: 2840492 De Jonckheere JF, et al. On the identity of the amoeboflagellates Didascalus thorntoni and Adelphamoeba galeacystis. J. Eukaryot. Microbiol. 44: 52-54, 1997. PubMed: 9172832 John DTOpportunistically pathogenic free-living amebaeIn: John DTParasitic protozoa2nd ed.3San DiegoAcademic Presspp. 143-246, 1993 Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 |
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| 产品名称： | Tetramitis thorntoni (Singh) Brown and De Jonckheere |
|---|---|
| 商品货号： | TS163504 |
| Deposited As： | Naegleria thorntoni (Singh) Page |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | farmyard soil, England, 1950 (?) |
| Product Format： | freeze-dried |
| Type Strain： | no |
| Comments： | Zymodeme Th-1 Maximum temperature tolerance 37C Circular ribosomal RNA genes Transfer of species back to Didasculus based upon SSUrDNA sequence review |
| Medium： | ATCC® Medium 997: Fresh water ameba medium |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Escherichia coli ATCC 11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Subcultivation： | Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Name of Depositor： | W Balamuth |
| Chain of Custody： | ATCC < |
| References： | Clark CG, Cross GA. Circular ribosomal RNA genes are a general feature of schizopyrenid amoebae. J. Protozool. 35: 326-329, 1988. PubMed: 2840492 De Jonckheere JF, et al. On the identity of the amoeboflagellates Didascalus thorntoni and Adelphamoeba galeacystis. J. Eukaryot. Microbiol. 44: 52-54, 1997. PubMed: 9172832 John DTOpportunistically pathogenic free-living amebaeIn: John DTParasitic protozoa2nd ed.3San DiegoAcademic Presspp. 143-246, 1993 Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158 |
