uPAR/Bluescript-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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uPAR/Bluescript
uPAR/Bluescript
规格:
货期:
编号:TS163535
品牌:Testobio
产品名称: uPAR/Bluescript
商品货号: TS163535
Designations: uPAR/Bluescript
Species: Homo sapiens, human
Depositors: E Kruithof
Vector:
Construct size (kb): 4.099999904632568
Insert:
DNA: cDNA
Insert lengths(kb): 1.11300003528595
Tissue: fibroblast GM637 cell line, SV40-transformed
Gene product: plasminogen activator, urokinase, receptor(activation antigen Mo3, urokinase plasminogen activator surface receptor) Mo3
Insert Size (kb): 1.113
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--3.0, 1.l; PstI--3.8, 0.3; HindIII--4.1; BamHI--3.5, 0.6; SmaI--4.1.
The insert contains the following restriction sites (nt position according to the published sequence): PstI--181, 449; BamHI--498, 1083; SmaI--576.
Corresponds to nt 31 - 1144 of the published sequence, and contains the complete coding region (nt 47 - 1054).
Transcription from the T7 promoter gives the sense strand.
References:

Roldan AL, et al. Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis. EMBO J. 9: 467-474, 1990. PubMed: 1689240

Egbert Kruithof, personal communication

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uPAR/Bluescript

  • 货号: TS163535
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  • 品牌 : TESTOBIO
产品名称: uPAR/Bluescript
商品货号: TS163535
Designations: uPAR/Bluescript
Species: Homo sapiens, human
Depositors: E Kruithof
Vector:
Construct size (kb): 4.099999904632568
Insert:
DNA: cDNA
Insert lengths(kb): 1.11300003528595
Tissue: fibroblast GM637 cell line, SV40-transformed
Gene product: plasminogen activator, urokinase, receptor(activation antigen Mo3, urokinase plasminogen activator surface receptor) Mo3
Insert Size (kb): 1.113
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--3.0, 1.l; PstI--3.8, 0.3; HindIII--4.1; BamHI--3.5, 0.6; SmaI--4.1.
The insert contains the following restriction sites (nt position according to the published sequence): PstI--181, 449; BamHI--498, 1083; SmaI--576.
Corresponds to nt 31 - 1144 of the published sequence, and contains the complete coding region (nt 47 - 1054).
Transcription from the T7 promoter gives the sense strand.
References:

Roldan AL, et al. Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis. EMBO J. 9: 467-474, 1990. PubMed: 1689240

Egbert Kruithof, personal communication

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