Plasmodium vivax (Grassi and Feletti) Labbe-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Plasmodium vivax (Grassi and Feletti) Labbe
Plasmodium vivax (Grassi and Feletti) Labbe
规格:
货期:
编号:TS163573
品牌:Testobio
产品名称： Plasmodium vivax (Grassi and Feletti) Labbe
商品货号： TS163573
Strain Designations： NICA
Application：
Emerging infectious disease research
Vector borne research
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
owl monkey, Nicaragua
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Comments：
infection in anopheline mosquitoes
transmission from monkey to man
Cryopreservation：

Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.

  1. To harvest parasites, inject host with ketamine (0.5-1.0ml depending on host size / weight).
  2. Exsanguinate via the femoral vein using a sterile syringe and Yaegers anticoagulant**, 1 volume anticoagulant to 4 volumes blood.xa0 Note: Unless there is an exchange transfusion, no more than 10% of estimated total blood volume should be collected over a 2 wk. period.
  3. Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube.
  4. Aspirate supernatant using sterile Pasteur pipette.
  5. Resuspend pellet gently in remaining supernatant.
  6. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet drop wise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. the remaining 4 volumes of glycerolyte and gently agitate.
  7. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.).
  8. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.

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Name of Depositor： National Institutes of Health
Chain of Custody：
ATCC <-- National Institutes of Health <-- CDC
References：

Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971.

Collins WE, et al. Transmission of four Central American strains of Plasmodium vivax from monkey to man. J. Parasitol. 58: 332-335, 1972. PubMed: 4623380

Collins WE, et al. Studies of comparative infectivity of fifteen strains of Plasmodium vivax to laboratory-reared anopheline mosquitoes, with special reference to Anopheles culicifacies. J. Parasitol. 72: 521-524, 1986. PubMed: 3537255

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Plasmodium vivax (Grassi and Feletti) Labbe

  • 货号: TS163573
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Plasmodium vivax (Grassi and Feletti) Labbe
商品货号： TS163573
Strain Designations： NICA
Application：
Emerging infectious disease research
Vector borne research
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
owl monkey, Nicaragua
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Comments：
infection in anopheline mosquitoes
transmission from monkey to man
Cryopreservation：

Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.

  1. To harvest parasites, inject host with ketamine (0.5-1.0ml depending on host size / weight).
  2. Exsanguinate via the femoral vein using a sterile syringe and Yaegers anticoagulant**, 1 volume anticoagulant to 4 volumes blood.xa0 Note: Unless there is an exchange transfusion, no more than 10% of estimated total blood volume should be collected over a 2 wk. period.
  3. Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube.
  4. Aspirate supernatant using sterile Pasteur pipette.
  5. Resuspend pellet gently in remaining supernatant.
  6. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet drop wise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. the remaining 4 volumes of glycerolyte and gently agitate.
  7. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.).
  8. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.

xa0xa0xa0xa0xa0xa0xa0

Name of Depositor： National Institutes of Health
Chain of Custody：
ATCC <-- National Institutes of Health <-- CDC
References：

Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971.

Collins WE, et al. Transmission of four Central American strains of Plasmodium vivax from monkey to man. J. Parasitol. 58: 332-335, 1972. PubMed: 4623380

Collins WE, et al. Studies of comparative infectivity of fifteen strains of Plasmodium vivax to laboratory-reared anopheline mosquitoes, with special reference to Anopheles culicifacies. J. Parasitol. 72: 521-524, 1986. PubMed: 3537255

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