| 产品名称： | CHB203 pCS806 |
|---|---|
| 商品货号： | TS163588 |
| Designations： | CHB203 pCS806 |
| GenBank Number： | J00139 |
| Species： | Homo sapiens, human |
| Depositors： | T Shimada |
| Vector： | Construct size (kb): 13.0 |
| Insert： | DNA: genomic Insert lengths(kb): 8.600000381469727 Tissue: fetal liver Gene product: dihydrofolate reductase DHFR Alleles: A1, A2 |
| Insert Size (kb)： | 8.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.5, 4.0, 3.4; SalI--14.0; PvuII--6.2, 4.2, 2.75; EcoRI--8.6, 4.4; HindIII--9.4, 3.7. Insert contains these sites in kb from 5 end: BglII--0.03, 3.3; AvaI--1.8; BglI--2.2, 7.5; SmaI--2.3; HindIII--3.5; PstI--4.6, 6.5, 7.3; XbaI--4.7; BamHI--5.0, 8.2; XbaI--6.3; SmaI/AvaI--7.2, 7.7; EcoRI--8.6. The insert includes exon 6 in a 1.2 kb SmaI/HindIII fragment. |
| References： | Anagnou NP, et al. Chromosomal organization of the human dihydrofolate reductase genes: dispersion, selective amplification, and a novel form of polymorphism. Proc. Natl. Acad. Sci. USA 81: 5170-5174, 1984. PubMed: 6089182 Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474 Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192 Chen MJ, et al. Intronless human dihydrofolate reductase genes are derived from processed RNA molecules. Proc. Natl. Acad. Sci. USA 79: 7435-7439, 1982. PubMed: 6961421 |
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| 产品名称： | CHB203 pCS806 |
|---|---|
| 商品货号： | TS163588 |
| Designations： | CHB203 pCS806 |
| GenBank Number： | J00139 |
| Species： | Homo sapiens, human |
| Depositors： | T Shimada |
| Vector： | Construct size (kb): 13.0 |
| Insert： | DNA: genomic Insert lengths(kb): 8.600000381469727 Tissue: fetal liver Gene product: dihydrofolate reductase DHFR Alleles: A1, A2 |
| Insert Size (kb)： | 8.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.5, 4.0, 3.4; SalI--14.0; PvuII--6.2, 4.2, 2.75; EcoRI--8.6, 4.4; HindIII--9.4, 3.7. Insert contains these sites in kb from 5 end: BglII--0.03, 3.3; AvaI--1.8; BglI--2.2, 7.5; SmaI--2.3; HindIII--3.5; PstI--4.6, 6.5, 7.3; XbaI--4.7; BamHI--5.0, 8.2; XbaI--6.3; SmaI/AvaI--7.2, 7.7; EcoRI--8.6. The insert includes exon 6 in a 1.2 kb SmaI/HindIII fragment. |
| References： | Anagnou NP, et al. Chromosomal organization of the human dihydrofolate reductase genes: dispersion, selective amplification, and a novel form of polymorphism. Proc. Natl. Acad. Sci. USA 81: 5170-5174, 1984. PubMed: 6089182 Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474 Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192 Chen MJ, et al. Intronless human dihydrofolate reductase genes are derived from processed RNA molecules. Proc. Natl. Acad. Sci. USA 79: 7435-7439, 1982. PubMed: 6961421 |
