42-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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42
42
规格:
货期:
编号:TS163772
品牌:Testobio
产品名称: 42
商品货号: TS163772
Designations: 42
GenBank Number:

M87857

Species: Homo sapiens, human
Vector:
Construct size (kb): 40.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJB8
Intact vector size: 5.400
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI EcoRI
Polylinker sites:
Construction: HomerI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references:
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LGALS2
Other gene symbols: GALBP
Genomic copy number: unique
Gene name: lectin, galactoside-binding, soluble, 2 (galectin 2)
Contains complete coding sequence?: U
Chromosome: 22; Localization: 22 q12-q13
Tissue: leukocyte
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 35.0
Cross references: DNA Seq. Acc.: M87857
DNA Seq. Acc.: M87858
DNA Seq. Acc.: M87859
DNA Seq. Acc.: M87860
Insert lengths(kb): 35.0
Tissue: leukocyte
Gene product: lectin, galactoside-binding, soluble, 2 (galectin 2) GALBP
Insert Size (kb): 35.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; BamHI/EcoRI--9.5, 7.5, 5.8, 3.0, 2.5, 1.85, 1.8, 1.6, 1.5, 1.05, 1.0, 0.75, 0.55, 0.55;
HindIII/EcoRI--12.5, 4.55, 3.6, 3.0, 2.9, 2.7, 2.5, 1.75, 1.7, 1.2, 1.08, 1.08, 1.0, 0.7, 0.55; KpnI/EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; SalI/EcoRI--15.0, 7.5, 5.0, 2.7, 2.5, 2.5, 1.85, 1.8, 1.05, 1.0, 0.55.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Contains the complete gene (4 exons).
References:

Lau YF, Kan YW. Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human alpha-globin cluster. Proc. Natl. Acad. Sci. USA 80: 5225-5229, 1983. PubMed: 6310566

Gitt MA, et al. Isolation and expression of a gene encoding L-14-II, a new human soluble lactose-binding lectin. J. Biol. Chem. 267: 10601-10606, 1992. PubMed: 1375225

Gitt MA, Barondes SH. Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30: 82-89, 1991. PubMed: 1988031

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42

  • 货号: TS163772
  • 好评
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  • 品牌 : TESTOBIO
产品名称: 42
商品货号: TS163772
Designations: 42
GenBank Number:

M87857

Species: Homo sapiens, human
Vector:
Construct size (kb): 40.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJB8
Intact vector size: 5.400
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI EcoRI
Polylinker sites:
Construction: HomerI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references:
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LGALS2
Other gene symbols: GALBP
Genomic copy number: unique
Gene name: lectin, galactoside-binding, soluble, 2 (galectin 2)
Contains complete coding sequence?: U
Chromosome: 22; Localization: 22 q12-q13
Tissue: leukocyte
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 35.0
Cross references: DNA Seq. Acc.: M87857
DNA Seq. Acc.: M87858
DNA Seq. Acc.: M87859
DNA Seq. Acc.: M87860
Insert lengths(kb): 35.0
Tissue: leukocyte
Gene product: lectin, galactoside-binding, soluble, 2 (galectin 2) GALBP
Insert Size (kb): 35.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; BamHI/EcoRI--9.5, 7.5, 5.8, 3.0, 2.5, 1.85, 1.8, 1.6, 1.5, 1.05, 1.0, 0.75, 0.55, 0.55;
HindIII/EcoRI--12.5, 4.55, 3.6, 3.0, 2.9, 2.7, 2.5, 1.75, 1.7, 1.2, 1.08, 1.08, 1.0, 0.7, 0.55; KpnI/EcoRI--15.0, 8.5, 7.5, 2.7, 1.85, 1.8, 1.05, 1.0, 0.55; SalI/EcoRI--15.0, 7.5, 5.0, 2.7, 2.5, 2.5, 1.85, 1.8, 1.05, 1.0, 0.55.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Contains the complete gene (4 exons).
References:

Lau YF, Kan YW. Versatile cosmid vectors for the isolation, expression, and rescue of gene sequences: studies with the human alpha-globin cluster. Proc. Natl. Acad. Sci. USA 80: 5225-5229, 1983. PubMed: 6310566

Gitt MA, et al. Isolation and expression of a gene encoding L-14-II, a new human soluble lactose-binding lectin. J. Biol. Chem. 267: 10601-10606, 1992. PubMed: 1375225

Gitt MA, Barondes SH. Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30: 82-89, 1991. PubMed: 1988031

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