VMM39

规格:

货期:

编号:TS163786

品牌:Testobio

产品名称：	VMM39
商品货号：	TS163786
Organism：	Homo sapiens, human
Tissue：	Melanoma, Lymph Node Metastasis
Cell Type：	Melanocyte
Product Format：	frozen
Morphology：	Epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Melanoma, Stage IIIC; malignant
Age：	71
Gender：	Male
Ethnicity：	Caucasian
Applications：	Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Derived from tumors taken from tumor-involved lymph nodes from patients at the University of Virginia
Clinical Data：	Primary Site: Right Forearm (Regressed Primary); Metastatic Site: Lymph Node (Right Axilla)
Antigen Expression：	Positives: Low GP100, Tyrosinase, Low MAGE-A1, MMP-1
HLA Typing：	A2,A3,B7,B44,BW4,BW6,DR15/13 xa0(HLA: A locus has been confirmed, other HLA typings may be serological and therefore not accurate)
Comments：	NRAS Mutation: Q61L Q61Rxa0 CDKN2A: wt BRAF: wt PDGFRA Mutation: D842V
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are for a T-75 flask; Adjust accordingly Remove and discard the cell culture medium from the flask. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37^oC. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the xa0 flask. Check the xa0cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting. Subculture as necessary. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing. Repeat when cells reach confluence.
Cryopreservation：	Fetal bovine serum, 90%; DMSO, 10%
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X,Y D5S818: 12 D13S317: 11,12 D7S820: 7,12 D16S539: 11,13 vWA: 16 TH01: 7 TPOX: 8,9 CSF1PO: 12
Sterility Tests：	Pass
Population Doubling Level (PDL)：	unknown
Population Doubling Capacity：	Unknown. 2 passages from 12/5/1997 frozen cell line stock, but it is not known how long the line was in culture after being established from tumor tissue obtained in 1995.
Name of Depositor：	Craig L. Slingluff, Jr. M.D.
Year of Origin：	1995
References：	Molhoek K, et al. Human melanoma cytolysis by combined inhibition of mammalian target of rapamycin and Vascular Endothelial Growth Factor/Vascular Endothelial Growth Factor Receptor-2. Cancer Res. 68: (11), 2008. PubMed: 18519701 Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344 Molhoek K, et al. Synergistic inhibition of human melanoma proliferation by combination treatment with B-Raf inhibitor BAY43-9006 and mTOR inhibitor Rapamycin. J. Trans. Med. 3(39): 2005. PubMed:16255777

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VMM39

货号: TS163786
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产品名称：	VMM39
商品货号：	TS163786
Organism：	Homo sapiens, human
Tissue：	Melanoma, Lymph Node Metastasis
Cell Type：	Melanocyte
Product Format：	frozen
Morphology：	Epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Melanoma, Stage IIIC; malignant
Age：	71
Gender：	Male
Ethnicity：	Caucasian
Applications：	Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	Derived from tumors taken from tumor-involved lymph nodes from patients at the University of Virginia
Clinical Data：	Primary Site: Right Forearm (Regressed Primary); Metastatic Site: Lymph Node (Right Axilla)
Antigen Expression：	Positives: Low GP100, Tyrosinase, Low MAGE-A1, MMP-1
HLA Typing：	A2,A3,B7,B44,BW4,BW6,DR15/13 xa0(HLA: A locus has been confirmed, other HLA typings may be serological and therefore not accurate)
Comments：	NRAS Mutation: Q61L Q61Rxa0 CDKN2A: wt BRAF: wt PDGFRA Mutation: D842V
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Volumes are for a T-75 flask; Adjust accordingly Remove and discard the cell culture medium from the flask. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37^oC. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the xa0 flask. Check the xa0cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting. Subculture as necessary. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing. Repeat when cells reach confluence.
Cryopreservation：	Fetal bovine serum, 90%; DMSO, 10%
Culture Conditions：	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
STR Profile：	Amelogenin: X,Y D5S818: 12 D13S317: 11,12 D7S820: 7,12 D16S539: 11,13 vWA: 16 TH01: 7 TPOX: 8,9 CSF1PO: 12
Sterility Tests：	Pass
Population Doubling Level (PDL)：	unknown
Population Doubling Capacity：	Unknown. 2 passages from 12/5/1997 frozen cell line stock, but it is not known how long the line was in culture after being established from tumor tissue obtained in 1995.
Name of Depositor：	Craig L. Slingluff, Jr. M.D.
Year of Origin：	1995
References：	Molhoek K, et al. Human melanoma cytolysis by combined inhibition of mammalian target of rapamycin and Vascular Endothelial Growth Factor/Vascular Endothelial Growth Factor Receptor-2. Cancer Res. 68: (11), 2008. PubMed: 18519701 Molhoek K, et al. Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res 21(4): 274–284, 2011. PubMed: 21654344 Molhoek K, et al. Synergistic inhibition of human melanoma proliferation by combination treatment with B-Raf inhibitor BAY43-9006 and mTOR inhibitor Rapamycin. J. Trans. Med. 3(39): 2005. PubMed:16255777