pCu424CTR1-CYC1-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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pCu424CTR1-CYC1
pCu424CTR1-CYC1
规格:
货期:
编号:TS163859
品牌:Testobio
产品名称: pCu424CTR1-CYC1
商品货号: TS163859
Designations: pCu424CTR1-CYC1
Depositors: S Labbe, DJ Thiele
Biosafety Level: 1
Vector Information:
Size (kb): 6.2280001640319820
Vector: pCu424CTR1-CYC1 (phagemid)
Promoters: Promoter for expression CTR1-CYC1
Construction: pRS424, CTR1-CYC1 promoter
Marker(s):TRP1,ampR
Construct size (kb): 6.228000164031982
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CTR1-CYC1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
Applications:
cloning vector
Comments:
Restriction digests of the vector give the following sizes (kb): SacI--6.2; SacI/SpeI--5.9, 0.4.
CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activated under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid.
References:

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

Related Products:
component of:ATCC 87778
Shipped: dried
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pCu424CTR1-CYC1

  • 货号: TS163859
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  • 品牌 : TESTOBIO
产品名称: pCu424CTR1-CYC1
商品货号: TS163859
Designations: pCu424CTR1-CYC1
Depositors: S Labbe, DJ Thiele
Biosafety Level: 1
Vector Information:
Size (kb): 6.2280001640319820
Vector: pCu424CTR1-CYC1 (phagemid)
Promoters: Promoter for expression CTR1-CYC1
Construction: pRS424, CTR1-CYC1 promoter
Marker(s):TRP1,ampR
Construct size (kb): 6.228000164031982
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CTR1-CYC1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
Applications:
cloning vector
Comments:
Restriction digests of the vector give the following sizes (kb): SacI--6.2; SacI/SpeI--5.9, 0.4.
CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activated under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid.
References:

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

Related Products:
component of:ATCC 87778
Shipped: dried
合作单位: