| 产品名称： | DT201 pDT201 |
|---|---|
| 商品货号： | TS163991 |
| Designations： | DT201 pDT201 |
| GenBank Number： | K01722 |
| Species： | Corynebacterium diphtheriae (Kruse) Lehmann and Neumann |
| Depositors： | President and Fellows of Harvard College, JR Murphy, President and Fellows of Harvard College |
| Vector： | Construct size (kb): 3.5
Name of vector: pUC8 Intact vector size: 2.700 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Construction: pBR322, Escherichia coli lacZalpha, M13mp8 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none |
| Insert： | Genome: Corynebacterium diphtheriae
Gene name: diphtheria toxin, fragment A
Contains complete coding sequence?: N Type of DNA: genomic Insert end: Sau3AI Insert end: Sau3AI Insert size (kb): 0.831 Cross references: DNA Seq. Acc.: K01722xa0Nucleotides 1-831 of the insert correspond toxa0nucleotides 58-889 of K01722. Insert lengths(kb): 0.831
Gene product: diphtheria toxin, fragment A |
| Insert Size (kb)： | 0.831 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Plasmid made from pUC8 and a small segment of the Corynebacteriophage beta tox-45 genome.Insert corresponds to nucleotides 57-888 of the genbank sequence K01722. |
| References： | Leong D, et al. Cloned fragment A of diphtheria toxin is expressed and secreted into the periplasmic space of Escherichia coli K12. Science 220: 515-517, 1983. PubMed: 6403984 Murphy JR. Hybrid protein. US Patent 4,675,382 dated Jun 23 1987 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | DT201 pDT201 |
|---|---|
| 商品货号： | TS163991 |
| Designations： | DT201 pDT201 |
| GenBank Number： | K01722 |
| Species： | Corynebacterium diphtheriae (Kruse) Lehmann and Neumann |
| Depositors： | President and Fellows of Harvard College, JR Murphy, President and Fellows of Harvard College |
| Vector： | Construct size (kb): 3.5
Name of vector: pUC8 Intact vector size: 2.700 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII Construction: pBR322, Escherichia coli lacZalpha, M13mp8 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none |
| Insert： | Genome: Corynebacterium diphtheriae
Gene name: diphtheria toxin, fragment A
Contains complete coding sequence?: N Type of DNA: genomic Insert end: Sau3AI Insert end: Sau3AI Insert size (kb): 0.831 Cross references: DNA Seq. Acc.: K01722xa0Nucleotides 1-831 of the insert correspond toxa0nucleotides 58-889 of K01722. Insert lengths(kb): 0.831
Gene product: diphtheria toxin, fragment A |
| Insert Size (kb)： | 0.831 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Plasmid made from pUC8 and a small segment of the Corynebacteriophage beta tox-45 genome.Insert corresponds to nucleotides 57-888 of the genbank sequence K01722. |
| References： | Leong D, et al. Cloned fragment A of diphtheria toxin is expressed and secreted into the periplasmic space of Escherichia coli K12. Science 220: 515-517, 1983. PubMed: 6403984 Murphy JR. Hybrid protein. US Patent 4,675,382 dated Jun 23 1987 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
