Bodo caudatus (Dujardin) Stein-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

你好，请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
你好,请登录   免费注册 | 收藏
联系电话: 0574-87917803
热门搜索: 金黄色葡萄球菌 铜绿假单胞菌 红菇 香菇
Bodo caudatus (Dujardin) Stein
Bodo caudatus (Dujardin) Stein
规格:
货期:
编号:TS164060
品牌:Testobio
产品名称： Bodo caudatus (Dujardin) Stein
商品货号： TS164060
Strain Designations： RCP
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Sediment near shore, Rock Creek, Rockville, MD, 1986
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Comments：
Forms stable cysts
Medium： ATCC® Medium 802: Sonneborns Paramecium medium
Growth Conditions：
Temperature: 25°C
Culture System: Xenic
Cryopreservation： Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.
  2. Adjust the concentration of cells to 2 x 106 to 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 20% (v/v) solution of sterile DMSO in fresh medium.
    1. Add 2.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube.
    2. Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 8.0 mL of ice cold medium.
    3. Invert several times to dissolve the DMSO.
    4. Allow to warm to room temperature.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 to 107 and 10% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min.
  5. Dispense in 0.5 mL aliquots into 1.0 mL toxa02.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate atxa0-1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. If frozen material is to be stored at temperatures between -130°C and -70°C the shelf life should be empirically tested, i.e., remove stored material at intervals to determine die-off rate.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the ampule to a level just above the surface of the frozen material. Do not agitate the ampule.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a T-25 tissue culture flask containing 10 mL of ATCC medium 802 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC® 700831).
  10. Incubate horizontally with the cap screwed on tightly at 25°C.
Name of Depositor： TA Nerad
Year of Origin： 1986
首页 > 产品中心 > 微生物培养 > 菌株 > null > Bodo caudatus (Dujardin) Stein

产品中心 Product Center

联系我们CONTACT US

  • 0574-87917803
    24小时服务热线
  • testobio@163.com
    销售邮箱
  • 浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
    地址

联系方式

  • 章丽敏
    17757489010
  • 朱静
    18067106081
  • 李晴
    18067408036
  • 李晶晶
    13375187189
  • 周雪
    13372109301

Bodo caudatus (Dujardin) Stein

  • 货号: TS164060
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Bodo caudatus (Dujardin) Stein
商品货号： TS164060
Strain Designations： RCP
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Sediment near shore, Rock Creek, Rockville, MD, 1986
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Comments：
Forms stable cysts
Medium： ATCC® Medium 802: Sonneborns Paramecium medium
Growth Conditions：
Temperature: 25°C
Culture System: Xenic
Cryopreservation： Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.
  2. Adjust the concentration of cells to 2 x 106 to 107/mL in fresh medium.
  3. While cells are centrifuging prepare a 20% (v/v) solution of sterile DMSO in fresh medium.
    1. Add 2.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube.
    2. Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 8.0 mL of ice cold medium.
    3. Invert several times to dissolve the DMSO.
    4. Allow to warm to room temperature.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 to 107 and 10% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min.
  5. Dispense in 0.5 mL aliquots into 1.0 mL toxa02.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the vials in a controlled rate freezing unit.xa0 From room temperature cool at -1°C/min to -40°C.xa0 If the freezing unit can compensate for the heat of fusion, maintain rate atxa0-1°C/min through the heat of fusion.xa0 At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. If frozen material is to be stored at temperatures between -130°C and -70°C the shelf life should be empirically tested, i.e., remove stored material at intervals to determine die-off rate.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the ampule to a level just above the surface of the frozen material. Do not agitate the ampule.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a T-25 tissue culture flask containing 10 mL of ATCC medium 802 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC® 700831).
  10. Incubate horizontally with the cap screwed on tightly at 25°C.
Name of Depositor： TA Nerad
Year of Origin： 1986
扫码关注小红书账号
关注我们
联系电话：0574-87917803
章丽敏：17757489010
朱静：18067106081
李晴：18067408036
李晶晶：13375187189
周雪：13372109301
邮箱：testobio@163.com
地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
在线留言
合作单位: