| 产品名称： | pMT2ADA-VWF |
|---|---|
| 商品货号： | TS164163 |
| Designations： | pMT2ADA-VWF |
| GenBank Number： | X04385 |
| Species： | Homo sapiens, human |
| Depositors： | Genetics Institute, Inc., RJ Kaufman, Genetics Institute, Inc. |
| Applications： | in suitable host, produces protein von Willebrand factor coagulation factor VIII VWF (von Willebrand factor) |
| Vector： | Construct size (kb): 13.83100032806397 |
| Insert： | DNA: cDNA Insert lengths(kb): 8.574999809265137 Gene product: von Willebrand factor(coagulation factor VIII VWF (von Willebrand factor)) VWF Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2 Target Gene: von Willebrand factor |
| Insert Size (kb)： | 8.575 |
| Media： | ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.0, 3.4, 2.8, 1.5, 0.6; EcoRI--7.6, 3.9, 2.5; BglII--4.4, 4.4, 3.5, 2.3. Contains the complete coding sequence (nt 100 to nt 8541), with the mature peptide encoded from nt 2389 to nt 8538. May be transfected into Chinese hamster ovary (CHO) cells in the ADA selective medium. The vector contains the SV40 ori, the adenovirus major late promoter and tripartite leader, the SV40 polyadenylation signal, the adenovirus VA I and VA II genes, murine adenosine deaminase (ADA) cDNA sequence, a DHFR cDNA sequence and pBR322 sequences. |
| References： | Kaufman RJ. Method for producing factor VIII:C and analogs. US Patent 5,198,349 dated Mar 30 1993 Kaufman RJ, et al. Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells. Proc. Natl. Acad. Sci. USA 83: 3136-3140, 1986. PubMed: 3486414 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pMT2ADA-VWF |
|---|---|
| 商品货号： | TS164163 |
| Designations： | pMT2ADA-VWF |
| GenBank Number： | X04385 |
| Species： | Homo sapiens, human |
| Depositors： | Genetics Institute, Inc., RJ Kaufman, Genetics Institute, Inc. |
| Applications： | in suitable host, produces protein von Willebrand factor coagulation factor VIII VWF (von Willebrand factor) |
| Vector： | Construct size (kb): 13.83100032806397 |
| Insert： | DNA: cDNA Insert lengths(kb): 8.574999809265137 Gene product: von Willebrand factor(coagulation factor VIII VWF (von Willebrand factor)) VWF Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2 Target Gene: von Willebrand factor |
| Insert Size (kb)： | 8.575 |
| Media： | ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.0, 3.4, 2.8, 1.5, 0.6; EcoRI--7.6, 3.9, 2.5; BglII--4.4, 4.4, 3.5, 2.3. Contains the complete coding sequence (nt 100 to nt 8541), with the mature peptide encoded from nt 2389 to nt 8538. May be transfected into Chinese hamster ovary (CHO) cells in the ADA selective medium. The vector contains the SV40 ori, the adenovirus major late promoter and tripartite leader, the SV40 polyadenylation signal, the adenovirus VA I and VA II genes, murine adenosine deaminase (ADA) cDNA sequence, a DHFR cDNA sequence and pBR322 sequences. |
| References： | Kaufman RJ. Method for producing factor VIII:C and analogs. US Patent 5,198,349 dated Mar 30 1993 Kaufman RJ, et al. Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells. Proc. Natl. Acad. Sci. USA 83: 3136-3140, 1986. PubMed: 3486414 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
