Unda schaefferi Sawyer-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Unda schaefferi Sawyer
Unda schaefferi Sawyer
规格:
货期:
编号:TS164298
品牌:Testobio
产品名称： Unda schaefferi Sawyer
商品货号： TS164298
Strain Designations： BSB-05190019
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Sediment core from Red Bank, University of Virginia LTER site, 1999
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain： no
Medium： ATCC® Medium 994: Marine ameba medium
Growth Conditions：
Temperature: 25°C
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 2.0 mL
Filtered artificial seawater (or similar), 8.0 mL

Harvest and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL sterile filtered artificial seawater and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 994.xa0 Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 25°C.
  10. Follow the protocol for maintenance of culture.
Name of Depositor： TK Sawyer
Year of Origin： 1999
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Unda schaefferi Sawyer

  • 货号: TS164298
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Unda schaefferi Sawyer
商品货号： TS164298
Strain Designations： BSB-05190019
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Sediment core from Red Bank, University of Virginia LTER site, 1999
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section
Type Strain： no
Medium： ATCC® Medium 994: Marine ameba medium
Growth Conditions：
Temperature: 25°C
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 2.0 mL
Filtered artificial seawater (or similar), 8.0 mL

Harvest and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL sterile filtered artificial seawater and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 994.xa0 Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 25°C.
  10. Follow the protocol for maintenance of culture.
Name of Depositor： TK Sawyer
Year of Origin： 1999
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