| 产品名称： | PYS-2 |
|---|---|
| 商品货号： | TS164450 |
| Organism： | Mus musculus, mouse |
| Tissue： | yolk sac; parietal endoderm |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | embryo |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | osteonectin (BM-40) |
| Cellular Products： | osteonectin (BM-40) |
| Comments： | SPARC is an extracellular Ca+2 binding glycoprotein, which is differentially associated with morphogeneisis, remodeling, cellular migration and proliferation PubMed; 2745554.
Exogenous SPARC, added to cells in culture is associated with profound changes in cell shape, caused rapid, partial detachment of a confluent monolayer, and inhibited spreading of newly plated cells PubMed; 2745554. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor： | EH Sage |
| Deposited As： | mouse |
| References： | Sage H, et al. SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix. J. Cell Biol. 109: 341-356, 1989. PubMed: 2745554 Basu A, et al. The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO. Mol. Cell. Biol. 17: 677-686, 1997. PubMed: 9001221 Satyamoorthy K, et al. Identification of an intronic enhancer that nullifies upstream repression of SPARC gene expression. Nucleic Acids Res. 25: 3169-3174, 1997. PubMed: 9224620 Lehman JM, et al. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma. J. Cell. Physiol. : 13-27, 1974. PubMed: 4858519 |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | PYS-2 |
|---|---|
| 商品货号： | TS164450 |
| Organism： | Mus musculus, mouse |
| Tissue： | yolk sac; parietal endoderm |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | embryo |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | osteonectin (BM-40) |
| Cellular Products： | osteonectin (BM-40) |
| Comments： | SPARC is an extracellular Ca+2 binding glycoprotein, which is differentially associated with morphogeneisis, remodeling, cellular migration and proliferation PubMed; 2745554.
Exogenous SPARC, added to cells in culture is associated with profound changes in cell shape, caused rapid, partial detachment of a confluent monolayer, and inhibited spreading of newly plated cells PubMed; 2745554. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor： | EH Sage |
| Deposited As： | mouse |
| References： | Sage H, et al. SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix. J. Cell Biol. 109: 341-356, 1989. PubMed: 2745554 Basu A, et al. The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO. Mol. Cell. Biol. 17: 677-686, 1997. PubMed: 9001221 Satyamoorthy K, et al. Identification of an intronic enhancer that nullifies upstream repression of SPARC gene expression. Nucleic Acids Res. 25: 3169-3174, 1997. PubMed: 9224620 Lehman JM, et al. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma. J. Cell. Physiol. : 13-27, 1974. PubMed: 4858519 |
