| 产品名称： | pLUC Phagemid in Escherichia coli HB101 |
|---|---|
| 商品货号： | TS164500 |
| Designations： | pLUC Phagemid in Escherichia coli HB101 |
| Depositors： | A Ward |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.40
Vector: pLUC (phagemid)
Promoters: Promoter lac
Construction: pSVOALdelta5, pBluescriptSK+
Marker(s):ampR
Construct size (kb): 5.40
Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1, f1 terminator: SV40 polyadenylation |
| Applications： | xa0promoter-cloning vector
xa0vector containing primer sites useful for sequencing
xa0vector permitting production of single-stranded DNA
xa0vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.4; HindIII--5.4; XhoI--5.4. The AUG start codon is 22bp downstream from the HindIII site within the luc gene. Differs from pLUCS (ATCC 77415) only in the polylinker. Permits beta-galactosidase blue/white color selection for identification of recombinants. The color is weaker than from the parent vector and transformed cells may need to be grown 18-24 hours at 37C. Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene. Constructed from pSVOALdelta5 by inserting a 2.6 kb BamHI fragment, containing the firefly (Photinus pyralis) luciferase gene and SV40 sequences into the BamHI site of pBluescriptSK+, followed by the removal of the 3 BamHI site. The order of the major features in the phagemid is: 3 lacZ - XhoI/MCS/BamHI - 5 luc 3 - SV40 small t intron - SV40 polyadenylation signal - NotI - SstII - 5 lacZ - pMB1 ori - ampR - f1 ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727 Caricasole A, Ward A. A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters. Gene 124: 139-140, 1993. PubMed: 8382654 Caricasole A, Ward A. Transactivation of mouse insulin-like growth factor II (TGF-II) gene promoters by the AP-1 complex. Nucleic Acids Res. 21: 1873-1879, 1993. PubMed: 8493103 |
| Shipped： | freeze-dried |
| Shipping Information： | Freeze dried phagemid in E. coli |
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| 产品名称： | pLUC Phagemid in Escherichia coli HB101 |
|---|---|
| 商品货号： | TS164500 |
| Designations： | pLUC Phagemid in Escherichia coli HB101 |
| Depositors： | A Ward |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.40
Vector: pLUC (phagemid)
Promoters: Promoter lac
Construction: pSVOALdelta5, pBluescriptSK+
Marker(s):ampR
Construct size (kb): 5.40
Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1, f1 terminator: SV40 polyadenylation |
| Applications： | xa0promoter-cloning vector
xa0vector containing primer sites useful for sequencing
xa0vector permitting production of single-stranded DNA
xa0vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.4; HindIII--5.4; XhoI--5.4. The AUG start codon is 22bp downstream from the HindIII site within the luc gene. Differs from pLUCS (ATCC 77415) only in the polylinker. Permits beta-galactosidase blue/white color selection for identification of recombinants. The color is weaker than from the parent vector and transformed cells may need to be grown 18-24 hours at 37C. Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene. Constructed from pSVOALdelta5 by inserting a 2.6 kb BamHI fragment, containing the firefly (Photinus pyralis) luciferase gene and SV40 sequences into the BamHI site of pBluescriptSK+, followed by the removal of the 3 BamHI site. The order of the major features in the phagemid is: 3 lacZ - XhoI/MCS/BamHI - 5 luc 3 - SV40 small t intron - SV40 polyadenylation signal - NotI - SstII - 5 lacZ - pMB1 ori - ampR - f1 ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727 Caricasole A, Ward A. A luciferase-reporter vector with blue-white selection for rapid subcloning and mutational analysis of eukaryotic promoters. Gene 124: 139-140, 1993. PubMed: 8382654 Caricasole A, Ward A. Transactivation of mouse insulin-like growth factor II (TGF-II) gene promoters by the AP-1 complex. Nucleic Acids Res. 21: 1873-1879, 1993. PubMed: 8493103 |
| Shipped： | freeze-dried |
| Shipping Information： | Freeze dried phagemid in E. coli |
