| 产品名称： | Tomato spotted wilt virus |
|---|---|
| 商品货号： | TS164520 |
| Deposited As： | Tomato spotted wilt tospovirus |
| Agent： | Tomato spotted wilt virus |
| Strain： | antiserum to nSM gene of TSWV strain L3 |
| Applications： | The recommended titer for Western blotting is 1:5000. Plant research |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | The nSM gene of the L3 isolate of TSWV was expressed using vector pET28a and bacterium E. coli HMS174 (DE3) and protein purified using His-column. Rabbits were inoculated with the purified recombinant protein. The crude serum was cross absorbed with lysed bacteria, and the IgG purified using a Protein A column. |
| Product Format： | frozen |
| Comments： | The nSM gene of the L3 isolate of TSWV was expressed using vector pET28a and bacterium E. coli HMS174 (DE3) and protein purified using His-column. Rabbits were inoculated with the purified recombinant protein. The crude serum was cross absorbed with lysed bacteria, and the IgG purified using a Protein A column. The antiserum is frozen with 50% glycerol and 0.05% sodium azide. The recommended titer for Western blotting is 1:5000. |
| Name of Depositor： | UB Gunasinghe |
| References： | Soellick TR, et al. The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins. Proc. Natl. Acad. Sci. USA 97: 2373-2378, 2000. PubMed: 10688879 |
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| 产品名称： | Tomato spotted wilt virus |
|---|---|
| 商品货号： | TS164520 |
| Deposited As： | Tomato spotted wilt tospovirus |
| Agent： | Tomato spotted wilt virus |
| Strain： | antiserum to nSM gene of TSWV strain L3 |
| Applications： | The recommended titer for Western blotting is 1:5000. Plant research |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | The nSM gene of the L3 isolate of TSWV was expressed using vector pET28a and bacterium E. coli HMS174 (DE3) and protein purified using His-column. Rabbits were inoculated with the purified recombinant protein. The crude serum was cross absorbed with lysed bacteria, and the IgG purified using a Protein A column. |
| Product Format： | frozen |
| Comments： | The nSM gene of the L3 isolate of TSWV was expressed using vector pET28a and bacterium E. coli HMS174 (DE3) and protein purified using His-column. Rabbits were inoculated with the purified recombinant protein. The crude serum was cross absorbed with lysed bacteria, and the IgG purified using a Protein A column. The antiserum is frozen with 50% glycerol and 0.05% sodium azide. The recommended titer for Western blotting is 1:5000. |
| Name of Depositor： | UB Gunasinghe |
| References： | Soellick TR, et al. The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins. Proc. Natl. Acad. Sci. USA 97: 2373-2378, 2000. PubMed: 10688879 |
