| 产品名称： | pKMY266 EcY5057, pKY266plasmid in E. coli |
|---|---|
| 商品货号： | TS164984 |
| Designations： | pKMY266 EcY5057, pKY266plasmid in E. coli |
| GenBank Number： | M65106 M95045 |
| Species： | Pseudomonas mendocina Palleroni |
| Depositors： | Amgen, Inc., K Yen, Amgen, Inc. |
| Applications： | contains sequence toluene-4-monooxygenase produces protein toluene-4-monooxygenase |
| Vector： | Construct size (kb): 45.40 |
| Insert： | DNA: genomic Insert lengths(kb): 20.4 Gene product: toluene-4-monooxygenase A tmoA |
| Insert Size (kb)： | 20.400 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--23.0, 16.0, 2.4; HindIII--27.5, 4.4, 2.9, 2.6, 2.1, 1.7, 1.1, 0.82; SacI--24.0, 10.0, 9.8. The complete coding sequence can also be isolated from partial digests of the clone as a 6.7 kb HindIII-BamHI fragment or a 4.7 kb HindIII-SmaI fragment. Insert contains the following restriction sites approximate kb from the 5 end): BamHI--18.1; BglI--13.3, 14.5; EcoRI--16.9; HindIII--14.0, 14.8, 19.3; PstI--14.3, 14.6; SacI--10.2; SalI--12.2, 12.8; SmaI--16.1; XhoI--15.3, 20.1. Insert contains two SacI fragments of 10.2 kb each. The downstream fragment encodes the six open reading frames involved in the expression of toluene-4-monooxygenase. |
| References： | Yen KM, et al. Bioconversions catalyzed by the toluene monooxygenase of Pseudomonas mendocina KR-1. US Patent 5,171,684 dated Dec 15 1992 Yen KM, et al. Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase. J. Bacteriol. 173: 5315-5327, 1991. PubMed: 1885512 Yen KM, Karl MR. Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase. J. Bacteriol. 174: 7253-7261, 1992. PubMed: 1429451 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pKMY266 EcY5057, pKY266plasmid in E. coli |
|---|---|
| 商品货号： | TS164984 |
| Designations： | pKMY266 EcY5057, pKY266plasmid in E. coli |
| GenBank Number： | M65106 M95045 |
| Species： | Pseudomonas mendocina Palleroni |
| Depositors： | Amgen, Inc., K Yen, Amgen, Inc. |
| Applications： | contains sequence toluene-4-monooxygenase produces protein toluene-4-monooxygenase |
| Vector： | Construct size (kb): 45.40 |
| Insert： | DNA: genomic Insert lengths(kb): 20.4 Gene product: toluene-4-monooxygenase A tmoA |
| Insert Size (kb)： | 20.400 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--23.0, 16.0, 2.4; HindIII--27.5, 4.4, 2.9, 2.6, 2.1, 1.7, 1.1, 0.82; SacI--24.0, 10.0, 9.8. The complete coding sequence can also be isolated from partial digests of the clone as a 6.7 kb HindIII-BamHI fragment or a 4.7 kb HindIII-SmaI fragment. Insert contains the following restriction sites approximate kb from the 5 end): BamHI--18.1; BglI--13.3, 14.5; EcoRI--16.9; HindIII--14.0, 14.8, 19.3; PstI--14.3, 14.6; SacI--10.2; SalI--12.2, 12.8; SmaI--16.1; XhoI--15.3, 20.1. Insert contains two SacI fragments of 10.2 kb each. The downstream fragment encodes the six open reading frames involved in the expression of toluene-4-monooxygenase. |
| References： | Yen KM, et al. Bioconversions catalyzed by the toluene monooxygenase of Pseudomonas mendocina KR-1. US Patent 5,171,684 dated Dec 15 1992 Yen KM, et al. Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase. J. Bacteriol. 173: 5315-5327, 1991. PubMed: 1885512 Yen KM, Karl MR. Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase. J. Bacteriol. 174: 7253-7261, 1992. PubMed: 1429451 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
