| 产品名称： | pVL941.GCRD21 |
|---|---|
| 商品货号： | TS165092 |
| Designations： | pVL941.GCRD21 |
| GenBank Number： | M11080 |
| Species： | Homo sapiens, human |
| Depositors： | Genzyme Corp., ML Bergh, Genzyme Corp. |
| Applications： | in another host, produces protein glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase |
| Vector： | Construct size (kb): 11.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.899999976158142 Gene product: polyhedrin GBA Target Gene: glucosidase, beta; acid |
| Insert Size (kb)： | 1.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of Escherichia coli DH5 alpha containing the plasmid. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--11.5; HindIII--4.0, 3.5, 1.9, 1.1, 1.0; PstI--11.0, 0.3; PvuII--3.5, 3.1, 2.4, 1.4, 0.7, 0.3. The glucocerebrosidase insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.83; PstI--0.27, 0.56; SalI--1.25; BamHI--1.48. Contains the complete coding sequence for glucocerebrosidase (nt 6-1556), plus 290 nt at the 3 end. Corresponds approximately to nt 490-2336 of the sequence record. pVL941 is a transfer vector encoding polyhedrin and containing its complete 5 non-coding regulator sequence. |
| References： | Rassmussen J, et al. Enzymatically active recombinant glucocerebrosidase. US Patent 5,236,838 dated Aug 17 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pVL941.GCRD21 |
|---|---|
| 商品货号： | TS165092 |
| Designations： | pVL941.GCRD21 |
| GenBank Number： | M11080 |
| Species： | Homo sapiens, human |
| Depositors： | Genzyme Corp., ML Bergh, Genzyme Corp. |
| Applications： | in another host, produces protein glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase |
| Vector： | Construct size (kb): 11.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.899999976158142 Gene product: polyhedrin GBA Target Gene: glucosidase, beta; acid |
| Insert Size (kb)： | 1.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of Escherichia coli DH5 alpha containing the plasmid. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--11.5; HindIII--4.0, 3.5, 1.9, 1.1, 1.0; PstI--11.0, 0.3; PvuII--3.5, 3.1, 2.4, 1.4, 0.7, 0.3. The glucocerebrosidase insert contains the following restriction sites (approximate kb from the 5 end): HindIII--0.83; PstI--0.27, 0.56; SalI--1.25; BamHI--1.48. Contains the complete coding sequence for glucocerebrosidase (nt 6-1556), plus 290 nt at the 3 end. Corresponds approximately to nt 490-2336 of the sequence record. pVL941 is a transfer vector encoding polyhedrin and containing its complete 5 non-coding regulator sequence. |
| References： | Rassmussen J, et al. Enzymatically active recombinant glucocerebrosidase. US Patent 5,236,838 dated Aug 17 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
