| 产品名称： | pE7 |
|---|---|
| 商品货号： | TS165390 |
| Designations： | pE7 |
| GenBank Number： | K01885 |
| Species： | Homo sapiens, human |
| Applications： | Codominant segregation of the BglII RFLP was observed in 7 families (32 individuals). For the HincII polymorphism, 9 constant bands smaller than 7.6 kb have been observed. |
| Vector： | Construct size (kb): 6.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.400000095367432 Tissue: epidermoid carcinoma A431 cell line Gene product: epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog) EGFR Alleles: A3, A3, B1, B1, B2, B2, A1, A1, A2, A2 |
| Insert Size (kb)： | 2.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): ClaI--4.4, 2.4; EcoRI--5.2, 1.7; HindIII--7.0. Codominant segregation of the BglII RFLP was observed in 7 families (32 individuals). This is an internal cDNA fragment encoding about 709 amino acids. The 3 end is determined by the ClaI site of the cDNA. For the HincII polymorphism, 9 constant bands smaller than 7.6 kb have been observed. Enzyme(s) not detecting polymorphism: MspI, TaqI, EcoRI. |
| References： | Motomura K, et al. A new BglII RFLP at the epidermal growth factor receptor locus (erbB-1) on chromosome 7. Nucleic Acids Res. 17: 2369, 1989. PubMed: 2565027 Merlino GT, et al. Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells. Mol. Cell. Biol. 5: 1722-1734, 1985. PubMed: 2991749 Xu YH, et al. Human epidermal growth factor receptor cDNA is homologous to a variety of RNAs overproduced in A431 carcinoma cells. Nature 309: 806-810, 1984. PubMed: 6330563 Korc M, et al. Enhanced expression of epidermal growth factor receptor correlates with alterations of chromosome 7 in human pancreatic cancer. Proc. Natl. Acad. Sci. USA 83: 5141-5144, 1986. PubMed: 3014534 Abrahamson G, Wainscoat JS. A new HincII polymorphism at the epidermal growth factor receptor locus on chromosome 7. Nucleic Acids Res. 18: 3104, 1990. PubMed: 1971927 |
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| 产品名称： | pE7 |
|---|---|
| 商品货号： | TS165390 |
| Designations： | pE7 |
| GenBank Number： | K01885 |
| Species： | Homo sapiens, human |
| Applications： | Codominant segregation of the BglII RFLP was observed in 7 families (32 individuals). For the HincII polymorphism, 9 constant bands smaller than 7.6 kb have been observed. |
| Vector： | Construct size (kb): 6.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.400000095367432 Tissue: epidermoid carcinoma A431 cell line Gene product: epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog) EGFR Alleles: A3, A3, B1, B1, B2, B2, A1, A1, A2, A2 |
| Insert Size (kb)： | 2.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): ClaI--4.4, 2.4; EcoRI--5.2, 1.7; HindIII--7.0. Codominant segregation of the BglII RFLP was observed in 7 families (32 individuals). This is an internal cDNA fragment encoding about 709 amino acids. The 3 end is determined by the ClaI site of the cDNA. For the HincII polymorphism, 9 constant bands smaller than 7.6 kb have been observed. Enzyme(s) not detecting polymorphism: MspI, TaqI, EcoRI. |
| References： | Motomura K, et al. A new BglII RFLP at the epidermal growth factor receptor locus (erbB-1) on chromosome 7. Nucleic Acids Res. 17: 2369, 1989. PubMed: 2565027 Merlino GT, et al. Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells. Mol. Cell. Biol. 5: 1722-1734, 1985. PubMed: 2991749 Xu YH, et al. Human epidermal growth factor receptor cDNA is homologous to a variety of RNAs overproduced in A431 carcinoma cells. Nature 309: 806-810, 1984. PubMed: 6330563 Korc M, et al. Enhanced expression of epidermal growth factor receptor correlates with alterations of chromosome 7 in human pancreatic cancer. Proc. Natl. Acad. Sci. USA 83: 5141-5144, 1986. PubMed: 3014534 Abrahamson G, Wainscoat JS. A new HincII polymorphism at the epidermal growth factor receptor locus on chromosome 7. Nucleic Acids Res. 18: 3104, 1990. PubMed: 1971927 |
