Naegleria morganensis Dobson et al.-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Naegleria morganensis Dobson et al.
Naegleria morganensis Dobson et al.
规格:
货期:
编号:TS165426
品牌:Testobio
产品名称： Naegleria morganensis Dobson et al.
商品货号： TS165426
Deposited As： Naegleria sp.
Strain Designations： SWL NG-236
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Fresh water, River Murray, Morgan, Australia, 1984
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： yes
Comments：
Zymodeme 44-2
Equals NG-236, maximum temperature tolerance 44C
Medium： ATCC® Medium 997: Fresh water ameba medium
ATCC® Medium 711: PYB
Growth Conditions：
Temperature: 25°C
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Pages Balanced Salt Solution (or similar), 8.5 mL


Harvest and Preservationxa0
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL ATCC medium 1323 (Pages Balanced Salt Solution) and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 997.xa0 Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 25°C.
  10. Follow the protocol for maintenance of culture.
Name of Depositor： BS Robinson
Year of Origin： 1984
References：

Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158

Dobson PJ, et al. New thermophilic Naegleria species (Heterolobosea: Vahlkampfiidae) from Australia and Asia: allozyme, morphometric and physiological characterization. Acta Protozool. 36: 261-271, 1997.

type strain

De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.

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Naegleria morganensis Dobson et al.

  • 货号: TS165426
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Naegleria morganensis Dobson et al.
商品货号： TS165426
Deposited As： Naegleria sp.
Strain Designations： SWL NG-236
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Fresh water, River Murray, Morgan, Australia, 1984
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： yes
Comments：
Zymodeme 44-2
Equals NG-236, maximum temperature tolerance 44C
Medium： ATCC® Medium 997: Fresh water ameba medium
ATCC® Medium 711: PYB
Growth Conditions：
Temperature: 25°C
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Pages Balanced Salt Solution (or similar), 8.5 mL


Harvest and Preservationxa0
  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest cells from a culture which is at or near peak density by adding 5 mL ATCC medium 1323 (Pages Balanced Salt Solution) and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites.
  3. Adjust the concentration of cells to at least 2 x 106/mL in fresh medium.
  4. Mix the cell preparation and the cryoprotective solution in equal portions.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 997.xa0 Distribute the material evenly over the plate using a spread bar.
  9. Wrap the entire edge of the plate with parafilm and incubate upright at 25°C.
  10. Follow the protocol for maintenance of culture.
Name of Depositor： BS Robinson
Year of Origin： 1984
References：

Adams M, et al. A genetic approach to species criteria in the amoeba genus Naegleria using allozyme electrophoresis. Int. J. Parasitol. 19: 823-834, 1989. PubMed: 2635158

Dobson PJ, et al. New thermophilic Naegleria species (Heterolobosea: Vahlkampfiidae) from Australia and Asia: allozyme, morphometric and physiological characterization. Acta Protozool. 36: 261-271, 1997.

type strain

De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.

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