Sapocribrum chincoteaguense-质粒载体-ATCC-DSM-CCUG-泰斯拓生物

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Sapocribrum chincoteaguense
Sapocribrum chincoteaguense
规格:
货期:
编号:TS165704
品牌:Testobio
产品名称： Sapocribrum chincoteaguense
商品货号： TS165704
Deposited As： Sexangularia sp.
Strain Designations： Chinc5
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Moist soil from mud flat approximately 800 yards from the ocean, Chincoteague, VA, 2002
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Medium： ATCC® Medium 1525: Seawater 802 medium
Growth Conditions：
Temperature: 25°C
Culture System:xa0Grown with Enterobacter aerogenesxa0(ATCC® 13048™) and mixed bacteria
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 2.0 mL
Fresh growth medium w/o bacteria, 8.0 mL

Harvest and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO, the solution will warm up due to chemical heat.
  2. Harvest cells from a culture that is at or near peak density by centrifugation atxa0800 x g for 5 min.
  3. Adjust the concentration of cells to at least 2 x 107/mL in fresh medium.
  4. Mix the cell preparation and 20% (v/v) DMSO in equal portions. The final concentration will be
    1xa0x 107 cells/mL and 10% DMSO. The time from the mixing of the cell preparation andxa0cryoprotective solution to the start of the freezing process should be no less than 15 min. andxa0no more than 60 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0xa0mL sterile plastic screw-cappedxa0cryules (specialxa0plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature, cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C, plunge ampules into liquid nitrogen. Alternatively,xa0place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in thisxa0apparatus is approximately -1°C/min.)
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state, place the vial in a 35°C water bath. Immersexa0the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0Immediately after thawing, do not leave in water bath; aseptically remove the contents of thexa0ampule and inoculate into a T-25xa0tissue culture flask containing 10 mL ATCC medium 1525xa0bacterized with Enterobacter
    aerogenes     (ATCC® 13048™).
  9. Incubate at 25°C with the cap screwed on tightly.
  10. Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 mLxa0to 10.0 mL of bacterized ATCC medium 1525.
  11. Follow the protocol for maintenance of culture.
Name of Depositor： TA Nerad
References：

Thomas A Nerad, personal communication

Lahr DJ, et al. Sapocribrum chincoteaguense n. gen. n. sp.: A Small, Scale-bearing Amoebozoan with Flabellinid Affinities. J Eukaryot Microbiol doi: 10.1111/jeu.12199 Epub ahead of print, 2014. PubMed: 25515047

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Sapocribrum chincoteaguense

  • 货号: TS165704
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Sapocribrum chincoteaguense
商品货号： TS165704
Deposited As： Sexangularia sp.
Strain Designations： Chinc5
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Moist soil from mud flat approximately 800 yards from the ocean, Chincoteague, VA, 2002
Product Format： frozen
Storage Conditions： Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures:
2-8°C

Live Cultures:
See Protocols section for handling information
Type Strain： no
Medium： ATCC® Medium 1525: Seawater 802 medium
Growth Conditions：
Temperature: 25°C
Culture System:xa0Grown with Enterobacter aerogenesxa0(ATCC® 13048™) and mixed bacteria
Cryopreservation： Reagents
Cryoprotective Solution
DMSO, 2.0 mL
Fresh growth medium w/o bacteria, 8.0 mL

Harvest and Preservation
  1. Mix the components in the order listed. When the medium is added to the DMSO, the solution will warm up due to chemical heat.
  2. Harvest cells from a culture that is at or near peak density by centrifugation atxa0800 x g for 5 min.
  3. Adjust the concentration of cells to at least 2 x 107/mL in fresh medium.
  4. Mix the cell preparation and 20% (v/v) DMSO in equal portions. The final concentration will be
    1xa0x 107 cells/mL and 10% DMSO. The time from the mixing of the cell preparation andxa0cryoprotective solution to the start of the freezing process should be no less than 15 min. andxa0no more than 60 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0xa0mL sterile plastic screw-cappedxa0cryules (specialxa0plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature, cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C, plunge ampules into liquid nitrogen. Alternatively,xa0place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in thisxa0apparatus is approximately -1°C/min.)
  7. Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state, place the vial in a 35°C water bath. Immersexa0the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0Immediately after thawing, do not leave in water bath; aseptically remove the contents of thexa0ampule and inoculate into a T-25xa0tissue culture flask containing 10 mL ATCC medium 1525xa0bacterized with Enterobacter
    aerogenes     (ATCC® 13048™).
  9. Incubate at 25°C with the cap screwed on tightly.
  10. Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 mLxa0to 10.0 mL of bacterized ATCC medium 1525.
  11. Follow the protocol for maintenance of culture.
Name of Depositor： TA Nerad
References：

Thomas A Nerad, personal communication

Lahr DJ, et al. Sapocribrum chincoteaguense n. gen. n. sp.: A Small, Scale-bearing Amoebozoan with Flabellinid Affinities. J Eukaryot Microbiol doi: 10.1111/jeu.12199 Epub ahead of print, 2014. PubMed: 25515047

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