p35-1
p35-1
规格:
货期:
编号:TS168108
品牌:Testobio
| 产品名称： | p35-1 |
|---|---|
| 商品货号： | TS168108 |
| Designations： | p35-1 |
| GenBank Number： | M13509 |
| Species： | Homo sapiens, human |
| Depositors： | W Stetler-Stevenson |
| Vector： | Construct size (kb): 4.96999979019165 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pcD-X Intact vector size: 2.800 Type of vector: plasmid Cloning sites: Polylinker sites: Features (with orientation and position when available): |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MMP1 Other gene symbols: CLG Gene name: matrix metalloproteinase 1 (interstitial collagenase) Contains complete coding sequence?: Y Chromosome: 11; Localization: 11 q21-q22 Strain: A2058 melanoma cell line Tissue: brain metastasis Type of DNA: cDNA Insert 5 end: Modification: GC Insert 3 end: Modification: AT Insert size (kb): 2.2 Cross references: DNA Seq. Acc.: M13509 Insert lengths(kb): 2.200000047683716 Tissue: brain metastasis Gene product: matrix metalloproteinase 1 (interstitial collagenase)(collagenase, epidermolysis bullosa, dystrophic, (autosomal recessive), collagenase, fibroblast, collagenase, interstitial) CLG |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.45, 0.75; EcoRI--5.3; XhoI--3.0, 2.3; SacI--5.3; PstI--2.5, 1.5, 1.3. The insert contains the following restriction sites (bp from the 5 end of the cDNA sequence): BamHI--1290; EcoRI--274; HincII--1951; SacI--1010; PstI--1468; XbaI--138, 1570. The insert sequence is essentially identical to that reported for fibroblast collagenase except for noted changes. Contains the complete coding sequence (nt 68-1478 of the cDNA). The 2.2 kb XhoI fragment includes some sequence from the vector: 175 bp at the 5 end and 50 bp at the 3 end. |
| References： | Templeton NS, et al. Cloning and characterization of human tumor cell interstitial collagenase. Cancer Res. 50: 5431-5437, 1990. PubMed: 2167156 |
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| 产品名称： | p35-1 |
|---|---|
| 商品货号： | TS168108 |
| Designations： | p35-1 |
| GenBank Number： | M13509 |
| Species： | Homo sapiens, human |
| Depositors： | W Stetler-Stevenson |
| Vector： | Construct size (kb): 4.96999979019165 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pcD-X Intact vector size: 2.800 Type of vector: plasmid Cloning sites: Polylinker sites: Features (with orientation and position when available): |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MMP1 Other gene symbols: CLG Gene name: matrix metalloproteinase 1 (interstitial collagenase) Contains complete coding sequence?: Y Chromosome: 11; Localization: 11 q21-q22 Strain: A2058 melanoma cell line Tissue: brain metastasis Type of DNA: cDNA Insert 5 end: Modification: GC Insert 3 end: Modification: AT Insert size (kb): 2.2 Cross references: DNA Seq. Acc.: M13509 Insert lengths(kb): 2.200000047683716 Tissue: brain metastasis Gene product: matrix metalloproteinase 1 (interstitial collagenase)(collagenase, epidermolysis bullosa, dystrophic, (autosomal recessive), collagenase, fibroblast, collagenase, interstitial) CLG |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.45, 0.75; EcoRI--5.3; XhoI--3.0, 2.3; SacI--5.3; PstI--2.5, 1.5, 1.3. The insert contains the following restriction sites (bp from the 5 end of the cDNA sequence): BamHI--1290; EcoRI--274; HincII--1951; SacI--1010; PstI--1468; XbaI--138, 1570. The insert sequence is essentially identical to that reported for fibroblast collagenase except for noted changes. Contains the complete coding sequence (nt 68-1478 of the cDNA). The 2.2 kb XhoI fragment includes some sequence from the vector: 175 bp at the 5 end and 50 bp at the 3 end. |
| References： | Templeton NS, et al. Cloning and characterization of human tumor cell interstitial collagenase. Cancer Res. 50: 5431-5437, 1990. PubMed: 2167156 |
