| 产品名称： | pBSE36 |
|---|---|
| 商品货号： | TS168732 |
| Designations： | pBSE36 |
| GenBank Number： | M34077 |
| Species： | tobacco mild green mosaic tobamovirus (TMGMV) |
| Depositors： | F Garcia-Arenal |
| Vector： | Construct size (kb): 5.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.871999979019165 Gene product: genome, partial |
| Insert Size (kb)： | 1.872 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 2.0; PstI--3.4, 1.7; PvuII--2.8, 2.3. Insert corresponds to nt 1990 to nt 3862 of the genome. This includes coding sequences for the 183 kDa protein (exons nt 71 to nt 3403 and nt 3407 to nt 4900) and the 126 kDa protein (nt 71 to nt 3406). Linearizing with HindIII and transcription from the T7 promoter gives the minus (antisense) strand. Linearizing with EcoRI and transcription from the T3 promoter gives the plus (sense) strand. |
| Classification： | Tobamovirus |
| References： | Garcia-Arenal F. Sequence and structure at the genome 3 end of the U2-strain of tobacco mosaic virus, a histidine-accepting tobamovirus. Virology 167: 201-206, 1988. PubMed: 3188396 Dracopoli NC, et al. The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology 177: 553-558, 1990. PubMed: 2371769 F Garcia-Arenal, personal communication |
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| 产品名称： | pBSE36 |
|---|---|
| 商品货号： | TS168732 |
| Designations： | pBSE36 |
| GenBank Number： | M34077 |
| Species： | tobacco mild green mosaic tobamovirus (TMGMV) |
| Depositors： | F Garcia-Arenal |
| Vector： | Construct size (kb): 5.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.871999979019165 Gene product: genome, partial |
| Insert Size (kb)： | 1.872 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 2.0; PstI--3.4, 1.7; PvuII--2.8, 2.3. Insert corresponds to nt 1990 to nt 3862 of the genome. This includes coding sequences for the 183 kDa protein (exons nt 71 to nt 3403 and nt 3407 to nt 4900) and the 126 kDa protein (nt 71 to nt 3406). Linearizing with HindIII and transcription from the T7 promoter gives the minus (antisense) strand. Linearizing with EcoRI and transcription from the T3 promoter gives the plus (sense) strand. |
| Classification： | Tobamovirus |
| References： | Garcia-Arenal F. Sequence and structure at the genome 3 end of the U2-strain of tobacco mosaic virus, a histidine-accepting tobamovirus. Virology 167: 201-206, 1988. PubMed: 3188396 Dracopoli NC, et al. The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology 177: 553-558, 1990. PubMed: 2371769 F Garcia-Arenal, personal communication |
