| 产品名称： | pCLL2201 |
|---|---|
| 商品货号： | TS168758 |
| Designations： | pCLL2201 |
| GenBank Number： | M63556 |
| Species： | Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers |
| Depositors： | American Cyanamid Co., BA Rasmussen, American Cyanamid Co. |
| Applications： | in another host, produces protein metallo-beta-lactamase |
| Vector： | Construct size (kb): 10.10000038146973 |
| Insert： | DNA: genomic Insert lengths(kb): 6.5 Gene product: metallo-beta-lactamase ccrA Target Gene: metallo-beta-lactamase |
| Insert Size (kb)： | 6.5 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 3.5; HindIII--6.0, 1.9, 1.4, 0.8; PstI--6.3, 3.8; KpnI--10.1. The insert contains the following restriction sites (approximate kb from the end upstream of ccrA): PstI--0.5, MboI--0.9, 1.9; KpnI--1.2; HindIII--3.6, 5.4. The 941 bp MboI fragment contains the complete gene, with 150 bp 5 and 50 bp 3 flanking sequence. The sequence of most of this fragment has been reported. The vector is a derivative of pMK16 deleted for the carboxy terminal half of the tetR marker and has had the small EcoRI/BamHI fragment replaced with the MCS-containing EcoRI/BamHI fragment from plink 322. |
| References： | Rasmussen BA, et al. Escherichia coli chromosomal mutations that permit direct cloning of the Bacteroides fragilis metallo-beta-lactamase gene, ccrA. Mol. Microbiol. 5: 1211-1219, 1991. PubMed: 1956298 Rasmussen BA, et al. Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636. Antimicrob. Agents Chemother. 34: 1590-1592, 1990. PubMed: 2121094 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pCLL2201 |
|---|---|
| 商品货号： | TS168758 |
| Designations： | pCLL2201 |
| GenBank Number： | M63556 |
| Species： | Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers |
| Depositors： | American Cyanamid Co., BA Rasmussen, American Cyanamid Co. |
| Applications： | in another host, produces protein metallo-beta-lactamase |
| Vector： | Construct size (kb): 10.10000038146973 |
| Insert： | DNA: genomic Insert lengths(kb): 6.5 Gene product: metallo-beta-lactamase ccrA Target Gene: metallo-beta-lactamase |
| Insert Size (kb)： | 6.5 |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 3.5; HindIII--6.0, 1.9, 1.4, 0.8; PstI--6.3, 3.8; KpnI--10.1. The insert contains the following restriction sites (approximate kb from the end upstream of ccrA): PstI--0.5, MboI--0.9, 1.9; KpnI--1.2; HindIII--3.6, 5.4. The 941 bp MboI fragment contains the complete gene, with 150 bp 5 and 50 bp 3 flanking sequence. The sequence of most of this fragment has been reported. The vector is a derivative of pMK16 deleted for the carboxy terminal half of the tetR marker and has had the small EcoRI/BamHI fragment replaced with the MCS-containing EcoRI/BamHI fragment from plink 322. |
| References： | Rasmussen BA, et al. Escherichia coli chromosomal mutations that permit direct cloning of the Bacteroides fragilis metallo-beta-lactamase gene, ccrA. Mol. Microbiol. 5: 1211-1219, 1991. PubMed: 1956298 Rasmussen BA, et al. Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636. Antimicrob. Agents Chemother. 34: 1590-1592, 1990. PubMed: 2121094 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
