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Rhizamoeba sp.
Rhizamoeba sp.
规格:
货期:
编号:TS169342
品牌:Testobio
产品名称: Rhizamoeba sp.
商品货号: TS169342
Strain Designations: BSJ-04190019
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
sediment, saltwater
Beaufort North Carolina, United States
Product Format: frozen
Storage Conditions: liquid nitrogen vapor phase
Type Strain: no
Medium: ATCC® Medium 1525: Seawater 802 medium
Growth Conditions:
Temperature: 20.0°C
Duration: xenic; Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831) and as a food source
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Subcultivation:
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Cryopreservation:
Storage temperature: liquid nitrogen vapor phase
Name of Depositor: JC Cole, TA Nerad
Special Collection: NSF - Protistology
References:

sediment from a man-made marsh island at the edge of a dredge spoilage site

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Rhizamoeba sp.

  • 货号: TS169342
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: Rhizamoeba sp.
商品货号: TS169342
Strain Designations: BSJ-04190019
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation:
sediment, saltwater
Beaufort North Carolina, United States
Product Format: frozen
Storage Conditions: liquid nitrogen vapor phase
Type Strain: no
Medium: ATCC® Medium 1525: Seawater 802 medium
Growth Conditions:
Temperature: 20.0°C
Duration: xenic; Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831) and as a food source
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Subcultivation:
Protocol:
  1. Scrape the bottom of the flask, quickly agitate and withdraw 0.2 ml of cell suspension.
  2. Inoculate into 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC 700831).
    Note: the amoebae settle very quickly, so the transfer must be done very quickly to be effective.
  3. Incubate the flask at 20C.
Interval: biweekly
Cryopreservation:
Storage temperature: liquid nitrogen vapor phase
Name of Depositor: JC Cole, TA Nerad
Special Collection: NSF - Protistology
References:

sediment from a man-made marsh island at the edge of a dredge spoilage site

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