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pSREBP-1a
pSREBP-1a
规格:
货期:
编号:TS169486
品牌:Testobio
产品名称: pSREBP-1a
商品货号: TS169486
Designations: pSREBP-1a
GenBank Number:

U00968

Species: Homo sapiens, human
Depositors: JL Goldstein
Vector:
Construct size (kb): 7.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: SalI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
marker(s): ampR
replicon: pMB1, f1
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SREBF1
Other gene symbols: SREBP1A
Gene name: sterol regulatory element binding transcription factor 1
Contains complete coding sequence?: Y
Chromosome: 17
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 4.2
Cross references: DNA Seq. Acc.: U00968
Insert lengths(kb): 4.199999809265137
Gene product: sterol regulatory element binding transcription factor 1( SREBF1 sterol regulatory element binding transcription factor 1, SREBP1, sterol regulatory element binding protein-1) SREBF1
Insert Size (kb): 4.200
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/SalI--4.2, 3.2; EcoRI--4.3, 2.9; KpnI--6.0, 1.1, 0.4; EcoRI/SalI--2.8, 2.7, 1.5; SacI--6.0, 0.7, 0.5.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.7; KpnI--5.6, 1.2, 0.34; BamHI/SalI--4.2, 2.9; EcoRI/SalI--2.9, 2.7, 1.5; EcoRI--Eco47III--4.4, 1.5, 1.2.
The insert contains the following restriction sites (kb from the 5 end): Eco47III--1.174; EcoRI--2.668; KpnI--2.685, 3.021.
Constructed with an EcoRI linker at the 5 end and an EcoRI(NotI) adaptor at the 3 end.
References:

Yokoyama C, et al. SREBP-1, a basic helix-loop-helix leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell 75: 187-197, 1993. PubMed: 8402897

Joseph L Goldstein, personal communication

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pSREBP-1a

  • 货号: TS169486
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询价
  • 品牌 : TESTOBIO
产品名称: pSREBP-1a
商品货号: TS169486
Designations: pSREBP-1a
GenBank Number:

U00968

Species: Homo sapiens, human
Depositors: JL Goldstein
Vector:
Construct size (kb): 7.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: SalI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
marker(s): ampR
replicon: pMB1, f1
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SREBF1
Other gene symbols: SREBP1A
Gene name: sterol regulatory element binding transcription factor 1
Contains complete coding sequence?: Y
Chromosome: 17
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 4.2
Cross references: DNA Seq. Acc.: U00968
Insert lengths(kb): 4.199999809265137
Gene product: sterol regulatory element binding transcription factor 1( SREBF1 sterol regulatory element binding transcription factor 1, SREBP1, sterol regulatory element binding protein-1) SREBF1
Insert Size (kb): 4.200
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/SalI--4.2, 3.2; EcoRI--4.3, 2.9; KpnI--6.0, 1.1, 0.4; EcoRI/SalI--2.8, 2.7, 1.5; SacI--6.0, 0.7, 0.5.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.7; KpnI--5.6, 1.2, 0.34; BamHI/SalI--4.2, 2.9; EcoRI/SalI--2.9, 2.7, 1.5; EcoRI--Eco47III--4.4, 1.5, 1.2.
The insert contains the following restriction sites (kb from the 5 end): Eco47III--1.174; EcoRI--2.668; KpnI--2.685, 3.021.
Constructed with an EcoRI linker at the 5 end and an EcoRI(NotI) adaptor at the 3 end.
References:

Yokoyama C, et al. SREBP-1, a basic helix-loop-helix leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell 75: 187-197, 1993. PubMed: 8402897

Joseph L Goldstein, personal communication

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