pTacNeo1.3 [CMCC 2297]
pTacNeo1.3 [CMCC 2297]
规格:
货期:
编号:TS169654
品牌:Testobio
| 产品名称： | pTacNeo1.3 CMCC 2297 |
|---|---|
| 商品货号： | TS169654 |
| Designations： | pTacNeo1.3 CMCC 2297 |
| Depositors： | JC Hunter-Cevera |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.4499998092651370 Vector: pTacNeo1.3 (plasmid) Promoters: Promoter tac Construction: pBR322, TAC promoter, lacZ, modified APH(3)-I Marker(s):ampR Construct size (kb): 5.449999809265137 Features: marker(s): ampR promoter: tac replicon: pMB1 |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.45; HindIII--5.45; EcoRI--5.45; PvuII--4.0, 1.5; PstI--3.1, 2.35. This is an expression vector capable of producing a beta-galactosidase: insert: APH(3)-I fusion protein. The start codon of the modified APH(3)-I is followed by the eleventh codon (serine) of APH(3)-I. Codons 2-10 have been deleted. The upstream translation of LacZ is out of frame with APH(3)-I. Sequences from the TAA stop codon of APH(3)-I to the immediate downstream PvuII site encode a portion of Tn601. Sequences from the PvuII site to the PstI site are derived from Tn5. The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 amino-terminal codons of beta-galactosidase, and a gene encoding a modified aminoglycoside phosphotransferase (3)-I (APH(3)-I). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Jennie C Hunter-Cevera, personal communication |
| Shipped： | freeze-dried |
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| 产品名称： | pTacNeo1.3 CMCC 2297 |
|---|---|
| 商品货号： | TS169654 |
| Designations： | pTacNeo1.3 CMCC 2297 |
| Depositors： | JC Hunter-Cevera |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.4499998092651370 Vector: pTacNeo1.3 (plasmid) Promoters: Promoter tac Construction: pBR322, TAC promoter, lacZ, modified APH(3)-I Marker(s):ampR Construct size (kb): 5.449999809265137 Features: marker(s): ampR promoter: tac replicon: pMB1 |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.45; HindIII--5.45; EcoRI--5.45; PvuII--4.0, 1.5; PstI--3.1, 2.35. This is an expression vector capable of producing a beta-galactosidase: insert: APH(3)-I fusion protein. The start codon of the modified APH(3)-I is followed by the eleventh codon (serine) of APH(3)-I. Codons 2-10 have been deleted. The upstream translation of LacZ is out of frame with APH(3)-I. Sequences from the TAA stop codon of APH(3)-I to the immediate downstream PvuII site encode a portion of Tn601. Sequences from the PvuII site to the PstI site are derived from Tn5. The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 amino-terminal codons of beta-galactosidase, and a gene encoding a modified aminoglycoside phosphotransferase (3)-I (APH(3)-I). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Jennie C Hunter-Cevera, personal communication |
| Shipped： | freeze-dried |
