| 产品名称： | HSE26.1 |
|---|---|
| 商品货号： | TS169961 |
| Designations： | HSE26.1 |
| GenBank Number： | J04071 |
| Species： | Homo sapiens, human |
| Depositors： | JA Trapani |
| Vector： | Construct size (kb): 3.799999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pIBI24 Intact vector size: 2.878 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI XhoI ApaI mLuI SphI HindIII Polylinker sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI XhoI ApaI mLuI SphI HindIII Construction: pEMBL Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GZMB Other gene symbols: CTLA1; CSPB; CCPI; CGL-1 Genomic copy number: unique Gene name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) Contains complete coding sequence?: Y Chromosome: 14; Localization: 14 q11.2 Tissue: T lymphocyte, activated Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 0.9 Cross references: DNA Seq. Acc.: J04071 Insert lengths(kb): 0.8999999761581421 Tissue: T lymphocyte, activated Gene product: cytotoxic serine protease B CCPI |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--2.05, 1.35, 0.66; EcoRI--3.15, 0.92; HindIII--3.2, 0.82; PstI--3.65, 0.39; PvuII--2.7, 0.76, 0.36, 0.20; SalI--3.95. Contains the complete coding sequence (nt 17-760). The insert contains the following restriction sites (approximate kb from the 5 end): PvuII--0.2, 0.35; ApaI--0.22; PstI--0.35; BglI--0.4; TaqI--0.56; HindIII--0.72. Detects genomic restriction fragments of the following sizes (approximate kb): human, BamHI--12; human, BglII--14; human, BclI--10; human, EcoRV--18; human, TaqI--3.5, 2.5; human, PstI--4.0, 2.2; mouse, PstI--4.0. |
| References： | Trapani JA, et al. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. Proc. Natl. Acad. Sci. USA 85: 6924-6928, 1988. PubMed: 3261871 Klein JL, et al. Genomic organization and chromosomal assignment for a serine protease gene (CSPB) expressed by human cytotoxic lymphocytes. Genomics 5: 110-117, 1989. PubMed: 2788607 |
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| 产品名称： | HSE26.1 |
|---|---|
| 商品货号： | TS169961 |
| Designations： | HSE26.1 |
| GenBank Number： | J04071 |
| Species： | Homo sapiens, human |
| Depositors： | JA Trapani |
| Vector： | Construct size (kb): 3.799999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pIBI24 Intact vector size: 2.878 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI XhoI ApaI mLuI SphI HindIII Polylinker sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI XhoI ApaI mLuI SphI HindIII Construction: pEMBL Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GZMB Other gene symbols: CTLA1; CSPB; CCPI; CGL-1 Genomic copy number: unique Gene name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) Contains complete coding sequence?: Y Chromosome: 14; Localization: 14 q11.2 Tissue: T lymphocyte, activated Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 0.9 Cross references: DNA Seq. Acc.: J04071 Insert lengths(kb): 0.8999999761581421 Tissue: T lymphocyte, activated Gene product: cytotoxic serine protease B CCPI |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--2.05, 1.35, 0.66; EcoRI--3.15, 0.92; HindIII--3.2, 0.82; PstI--3.65, 0.39; PvuII--2.7, 0.76, 0.36, 0.20; SalI--3.95. Contains the complete coding sequence (nt 17-760). The insert contains the following restriction sites (approximate kb from the 5 end): PvuII--0.2, 0.35; ApaI--0.22; PstI--0.35; BglI--0.4; TaqI--0.56; HindIII--0.72. Detects genomic restriction fragments of the following sizes (approximate kb): human, BamHI--12; human, BglII--14; human, BclI--10; human, EcoRV--18; human, TaqI--3.5, 2.5; human, PstI--4.0, 2.2; mouse, PstI--4.0. |
| References： | Trapani JA, et al. Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. Proc. Natl. Acad. Sci. USA 85: 6924-6928, 1988. PubMed: 3261871 Klein JL, et al. Genomic organization and chromosomal assignment for a serine protease gene (CSPB) expressed by human cytotoxic lymphocytes. Genomics 5: 110-117, 1989. PubMed: 2788607 |
