| 产品名称： | pYHT1 |
|---|---|
| 商品货号： | TS170182 |
| Designations： | pYHT1 |
| GenBank Number： | X58156 |
| Species： | Homo sapiens, human |
| Depositors： | J Cheng |
| Vector： | Construct size (kb): 9.899999618530273 |
| Insert： | DNA: genomic Insert lengths(kb): 3.700000047683716 Tissue: fibroblast GM3468 cell line Gene product: subtelomeric and telomeric repeats |
| Insert Size (kb)： | 3.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--7.2, 2.9; EcoRI--10.0, HindIII--5.2, 2.9, 1.8; XhoI--10.0; PstI--3.8, 2.4, 1.7, 1.3, 0.8. The insert contains the following restriction sites (kb from the EcoRI end): PvuII--0.617, 1.838, 2.447; BglII--0.693, 0.719, 0.801; KpnI--1.324, 3.248; PstI--1.613, 1.833, 2.617, 3.497. The KpnI/PstI fragment (probe A1, nt 3249-3497) and the PvuII/KpnI fragment (probe A2, nt 2448-3248) cross-hybridize to hamster DNA and detect a number of variable fragments in human DNA. A2 contains a CpG island. The PvuII fragment (probe B, nt 1838-2448) does not cross-hybridize to hamster DNA. The EcoRI/BglII fragment (probe C, nt 1-693) does not cross-hybridize to hamster DNA. It detects a 6.0 kb poly(A)+ RNA in HeLa cells. The Alu repeat extends from nt 1368 to 1695. Generated from yHT1 (a 5.5 kb EcoRI/telomeric fragment ligated to the EcoRI/BamHI fragment of pYAC4) by digesting the linear DNA with Bal31 and self-ligating. There are residual yeast/Tetrahymena telomeric repeats located next to the vector HindIII site which is 0.9 kb from the PstI site at nt 3499 of the insert. |
| References： | Cheng JF, et al. Structural and transcriptional analysis of a human subtelomeric repeat. Nucleic Acids Res. 19: 149-154, 1991. PubMed: 2011494 Cheng JF, et al. Isolation and characterization of a human telomere. Nucleic Acids Res. 17: 6109-6127, 1989. PubMed: 2549507 |
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| 产品名称： | pYHT1 |
|---|---|
| 商品货号： | TS170182 |
| Designations： | pYHT1 |
| GenBank Number： | X58156 |
| Species： | Homo sapiens, human |
| Depositors： | J Cheng |
| Vector： | Construct size (kb): 9.899999618530273 |
| Insert： | DNA: genomic Insert lengths(kb): 3.700000047683716 Tissue: fibroblast GM3468 cell line Gene product: subtelomeric and telomeric repeats |
| Insert Size (kb)： | 3.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--7.2, 2.9; EcoRI--10.0, HindIII--5.2, 2.9, 1.8; XhoI--10.0; PstI--3.8, 2.4, 1.7, 1.3, 0.8. The insert contains the following restriction sites (kb from the EcoRI end): PvuII--0.617, 1.838, 2.447; BglII--0.693, 0.719, 0.801; KpnI--1.324, 3.248; PstI--1.613, 1.833, 2.617, 3.497. The KpnI/PstI fragment (probe A1, nt 3249-3497) and the PvuII/KpnI fragment (probe A2, nt 2448-3248) cross-hybridize to hamster DNA and detect a number of variable fragments in human DNA. A2 contains a CpG island. The PvuII fragment (probe B, nt 1838-2448) does not cross-hybridize to hamster DNA. The EcoRI/BglII fragment (probe C, nt 1-693) does not cross-hybridize to hamster DNA. It detects a 6.0 kb poly(A)+ RNA in HeLa cells. The Alu repeat extends from nt 1368 to 1695. Generated from yHT1 (a 5.5 kb EcoRI/telomeric fragment ligated to the EcoRI/BamHI fragment of pYAC4) by digesting the linear DNA with Bal31 and self-ligating. There are residual yeast/Tetrahymena telomeric repeats located next to the vector HindIII site which is 0.9 kb from the PstI site at nt 3499 of the insert. |
| References： | Cheng JF, et al. Structural and transcriptional analysis of a human subtelomeric repeat. Nucleic Acids Res. 19: 149-154, 1991. PubMed: 2011494 Cheng JF, et al. Isolation and characterization of a human telomere. Nucleic Acids Res. 17: 6109-6127, 1989. PubMed: 2549507 |
