Oxytricha trifallax

Oxytricha trifallax

规格:

货期:

编号:TS170355

品牌:Testobio

产品名称：	Oxytricha trifallax
商品货号：	TS170355
Strain Designations：	SLC 89
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：	Unknown
Product Format：	frozen
Storage Conditions：	Frozen: -70°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Protocols Section
Type Strain：	no
Medium：	ATCC^® Medium 802: Sonneborns Paramecium medium
Growth Conditions：	Temperature: 20°C to 25°C Culture system: Xenic, with Enterobacter aerogenes ATCC 13048 and unidentified microalgae as food sources
Cryopreservation：	Reagents Cryoprotective Solution DMSO, 2.0 mLxa0 Fresh growth medium w/o bacteria, 8.0 mL Harvest and Preservation Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.xa0 Harvest cells from a culture that is at or near peak density by centrifugation at 125 x g for 5 min.xa0 Adjust the concentration of cells at least 1 x 10⁵/mL in fresh medium.xa0 Mix the cell preparation and the cryoprotective solution in equal portions.xa0 Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.xa0 Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.xa0 To establish a culture from the frozen state add 1.0 mL bacterized ATCC medium 802 to the frozen ampule and place it in a 35°C water bath. xa0Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0 Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 mL of bacterized ATCC medium 802.xa0 Incubate at 25°C with the cap on loosely.xa0 Once the culture is established, transfer 0.5 mL to 5.0 mL of bacterized ATCC medium 802.xa0 Follow the protocol for maintenance of culture.
Name of Depositor：	GA Herrick
Geographical Isolation：	unknown

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Oxytricha trifallax

货号: TS170355
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产品名称：	Oxytricha trifallax
商品货号：	TS170355
Strain Designations：	SLC 89
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：	Unknown
Product Format：	frozen
Storage Conditions：	Frozen: -70°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Protocols Section
Type Strain：	no
Medium：	ATCC^® Medium 802: Sonneborns Paramecium medium
Growth Conditions：	Temperature: 20°C to 25°C Culture system: Xenic, with Enterobacter aerogenes ATCC 13048 and unidentified microalgae as food sources
Cryopreservation：	Reagents Cryoprotective Solution DMSO, 2.0 mLxa0 Fresh growth medium w/o bacteria, 8.0 mL Harvest and Preservation Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.xa0 Harvest cells from a culture that is at or near peak density by centrifugation at 125 x g for 5 min.xa0 Adjust the concentration of cells at least 1 x 10⁵/mL in fresh medium.xa0 Mix the cell preparation and the cryoprotective solution in equal portions.xa0 Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.xa0 Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.xa0 To establish a culture from the frozen state add 1.0 mL bacterized ATCC medium 802 to the frozen ampule and place it in a 35°C water bath. xa0Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0 Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 mL of bacterized ATCC medium 802.xa0 Incubate at 25°C with the cap on loosely.xa0 Once the culture is established, transfer 0.5 mL to 5.0 mL of bacterized ATCC medium 802.xa0 Follow the protocol for maintenance of culture.
Name of Depositor：	GA Herrick
Geographical Isolation：	unknown

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