| 产品名称： | pTSV-NS5 |
|---|---|
| 商品货号： | TS170555 |
| Designations： | pTSV-NS5 |
| Species： | Tobacco streak virus |
| Depositors： | TJ Morris |
| Applications： | This clone is useful in hybridization tests for the detection of tobacco streak virus (necrotic shock) infection in Fragaria spp. |
| Vector： | Construct size (kb): 3.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.3199999928474426 Gene product: genome, partial |
| Insert Size (kb)： | 0.320 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.8, 0.34; BamHI--3.2; EcoRI--3.2. This clone is useful in hybridization tests for the detection of tobacco streak virus (necrotic shock) infection in Fragaria spp. Insert hybridizes with NS (necrotic shock) RNA 3 from strawberry and R RNA 3 from blackberry but not to R RNA 4 in northern blots of genomic RNA. Isolated from Fragaria vesca. cDNA was synthesized from virion genomic RNA, dC-tailed and cloned into the dG-tailed PstI site of pUC8. |
| Classification： | Ilarvirus |
| References： | Stenger DC, et al. Characterization and detection of the strawberry necrotic shock isolate of tobacco streak virus. Phytopathology 77: 1330-1337, 1987. |
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| 产品名称： | pTSV-NS5 |
|---|---|
| 商品货号： | TS170555 |
| Designations： | pTSV-NS5 |
| Species： | Tobacco streak virus |
| Depositors： | TJ Morris |
| Applications： | This clone is useful in hybridization tests for the detection of tobacco streak virus (necrotic shock) infection in Fragaria spp. |
| Vector： | Construct size (kb): 3.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.3199999928474426 Gene product: genome, partial |
| Insert Size (kb)： | 0.320 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.8, 0.34; BamHI--3.2; EcoRI--3.2. This clone is useful in hybridization tests for the detection of tobacco streak virus (necrotic shock) infection in Fragaria spp. Insert hybridizes with NS (necrotic shock) RNA 3 from strawberry and R RNA 3 from blackberry but not to R RNA 4 in northern blots of genomic RNA. Isolated from Fragaria vesca. cDNA was synthesized from virion genomic RNA, dC-tailed and cloned into the dG-tailed PstI site of pUC8. |
| Classification： | Ilarvirus |
| References： | Stenger DC, et al. Characterization and detection of the strawberry necrotic shock isolate of tobacco streak virus. Phytopathology 77: 1330-1337, 1987. |
