| 产品名称： | pWL102 DSM5715, WFD1005 |
|---|---|
| 商品货号： | TS171157 |
| Designations： | pWL102 DSM5715, WFD1005 |
| Depositors： | WF Doolittle |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.5000000000000000 Vector: pWL102 (plasmid) Construction: pHV2, pAT153, Haloferax volcanii DS2 Marker(s):ampR,mevR Construct size (kb): 10.5 Features: marker(s): ampR, mevR replicon: pHV2, pMB1 |
| Applications： | contains sequence 3-hydroxy-3-methylglutaryl-Coenzyme A reductase shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--11.0; HindIII--6.6, 4.1; KpnI/SphI--7.2, 3.6; PstI--7.6, 3.0; XbaI--11.0. The 3.5 kb SphI/KpnI fragment conferring mevinolin resistance has been sequenced. The mutation in the hmgA gene is at position -29 relative to the transcription start site, and results in an increased level of transcription. Shuttle vector with demonstrated ability to transform Haloferax volcanii, Haloferax mediterranei (ATCC 33500), Halobacterium halobium, Haloarcula hispanica (ATCC 33960), and Haloarcula vallismortis (ATCC 29715). The order of the major features in this plasmid is: NcoI - BamHI - HindIII - XbaI - KpnI - mevR - sphI - pMB1 ori - ampR - EcoRI - ClaI - HindIII - pHV2 fragment. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Brooun A, et al. Primary structure and functional analysis of the soluble transducer protein HtrXI in the archaeon Halobacterium salinrrium. J. Bacteriol. 179: 2963-2968, 1997. PubMed: 9139915 Cline SW, Doolittle WF. Transformation of members of the genus Haloarcula with shuttle vectors based on Halobacterium halobium and Haloferax volcanii plasmid replicons. J. Bacteriol. 174: 1076-1080, 1992. PubMed: 1732202 Lam WL, Doolittle WF. Mevinolin-resistant mutations identify a promoter and the gene for a eukaryote-like 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the archaebacterium Haloferax volcanii. J. Biol. Chem. 267: 5829-5834, 1992. PubMed: 1556098 Lam WL, Doolittle WF. Shuttle vectors for the archaebacterium Halobacterium volcanii. Proc. Natl. Acad. Sci. USA 86: 5478-5482, 1989. PubMed: 2748598 |
| Shipped： | frozen |
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| 产品名称： | pWL102 DSM5715, WFD1005 |
|---|---|
| 商品货号： | TS171157 |
| Designations： | pWL102 DSM5715, WFD1005 |
| Depositors： | WF Doolittle |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.5000000000000000 Vector: pWL102 (plasmid) Construction: pHV2, pAT153, Haloferax volcanii DS2 Marker(s):ampR,mevR Construct size (kb): 10.5 Features: marker(s): ampR, mevR replicon: pHV2, pMB1 |
| Applications： | contains sequence 3-hydroxy-3-methylglutaryl-Coenzyme A reductase shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--11.0; HindIII--6.6, 4.1; KpnI/SphI--7.2, 3.6; PstI--7.6, 3.0; XbaI--11.0. The 3.5 kb SphI/KpnI fragment conferring mevinolin resistance has been sequenced. The mutation in the hmgA gene is at position -29 relative to the transcription start site, and results in an increased level of transcription. Shuttle vector with demonstrated ability to transform Haloferax volcanii, Haloferax mediterranei (ATCC 33500), Halobacterium halobium, Haloarcula hispanica (ATCC 33960), and Haloarcula vallismortis (ATCC 29715). The order of the major features in this plasmid is: NcoI - BamHI - HindIII - XbaI - KpnI - mevR - sphI - pMB1 ori - ampR - EcoRI - ClaI - HindIII - pHV2 fragment. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Brooun A, et al. Primary structure and functional analysis of the soluble transducer protein HtrXI in the archaeon Halobacterium salinrrium. J. Bacteriol. 179: 2963-2968, 1997. PubMed: 9139915 Cline SW, Doolittle WF. Transformation of members of the genus Haloarcula with shuttle vectors based on Halobacterium halobium and Haloferax volcanii plasmid replicons. J. Bacteriol. 174: 1076-1080, 1992. PubMed: 1732202 Lam WL, Doolittle WF. Mevinolin-resistant mutations identify a promoter and the gene for a eukaryote-like 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the archaebacterium Haloferax volcanii. J. Biol. Chem. 267: 5829-5834, 1992. PubMed: 1556098 Lam WL, Doolittle WF. Shuttle vectors for the archaebacterium Halobacterium volcanii. Proc. Natl. Acad. Sci. USA 86: 5478-5482, 1989. PubMed: 2748598 |
| Shipped： | frozen |
