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cosmid51.22
cosmid51.22
规格:
货期:
编号:TS171198
品牌:Testobio
产品名称: cosmid51.22
商品货号: TS171198
Designations: cosmid51.22
GenBank Number:

L01962

Species: Homo sapiens, human
Vector:
Construct size (kb): 45.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pWE15
Intact vector size: 8.200
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, kanR, G418R
promoter: T3, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65279
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN4A
Gene name: sodium channel, voltage-gated, type IV, alpha polypeptide
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q23.1-q25.3
Tissue: lymphoblast
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 37.0
Cross references: DNA Seq. Acc.: L01962
DNA Seq. Acc.: L01963
DNA Seq. Acc.: L01964
DNA Seq. Acc.: L01965
DNA Seq. Acc.: L01966
DNA Seq. Acc.: L01967
DNA Seq. Acc.: L01968
DNA Seq. Acc.: L01969
DNA Seq. Acc.: L01970
DNA Seq. Acc.: L01971
DNA Seq. Acc.: L01972
DNA Seq. Acc.: L01973
DNA Seq. Acc.: L01974
DNA Seq. Acc.: L01975
DNA Seq. Acc.: L01976
DNA Seq. Acc.: L01977
DNA Seq. Acc.: L01978
DNA Seq. Acc.: L01979
DNA Seq. Acc.: L01980
DNA Seq. Acc.: L01981
DNA Seq. Acc.: L01982
DNA Seq. Acc.: L01983
Insert lengths(kb): 37.0
Tissue: lymphoblast
Gene product: sodium channel, voltage-gated, type IV, alpha polypeptide SCN4A
Insert Size (kb): 37.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--12.5, 10.5, 5.3, 5.0, 3.8 (doublet), 3.6, 0.8; BglII--15.5 (doublet), 12.0; EcoRI--20.0, 12.0, 8.8, 2.1; HindIII--14.5, 12.0, 7.1, 3.75, 3.6, 2.1;
SstI--13.5, 9.4, 5.6, 5.3, 3.4, 1.75, 1.35, 1.05, 0.86.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Extends from exon 1 into exon 24, from the 5 untranslated region into the 3 untranslated region.
References:

McClatchey AI, et al. The genomic structure of the human skeletal muscle sodium channel gene. Hum. Mol. Genet. 1: 521-527, 1992. PubMed: 1339144

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cosmid51.22

  • 货号: TS171198
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  • 品牌 : TESTOBIO
产品名称: cosmid51.22
商品货号: TS171198
Designations: cosmid51.22
GenBank Number:

L01962

Species: Homo sapiens, human
Vector:
Construct size (kb): 45.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pWE15
Intact vector size: 8.200
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, kanR, G418R
promoter: T3, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65279
Insert:
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN4A
Gene name: sodium channel, voltage-gated, type IV, alpha polypeptide
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q23.1-q25.3
Tissue: lymphoblast
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 37.0
Cross references: DNA Seq. Acc.: L01962
DNA Seq. Acc.: L01963
DNA Seq. Acc.: L01964
DNA Seq. Acc.: L01965
DNA Seq. Acc.: L01966
DNA Seq. Acc.: L01967
DNA Seq. Acc.: L01968
DNA Seq. Acc.: L01969
DNA Seq. Acc.: L01970
DNA Seq. Acc.: L01971
DNA Seq. Acc.: L01972
DNA Seq. Acc.: L01973
DNA Seq. Acc.: L01974
DNA Seq. Acc.: L01975
DNA Seq. Acc.: L01976
DNA Seq. Acc.: L01977
DNA Seq. Acc.: L01978
DNA Seq. Acc.: L01979
DNA Seq. Acc.: L01980
DNA Seq. Acc.: L01981
DNA Seq. Acc.: L01982
DNA Seq. Acc.: L01983
Insert lengths(kb): 37.0
Tissue: lymphoblast
Gene product: sodium channel, voltage-gated, type IV, alpha polypeptide SCN4A
Insert Size (kb): 37.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--12.5, 10.5, 5.3, 5.0, 3.8 (doublet), 3.6, 0.8; BglII--15.5 (doublet), 12.0; EcoRI--20.0, 12.0, 8.8, 2.1; HindIII--14.5, 12.0, 7.1, 3.75, 3.6, 2.1;
SstI--13.5, 9.4, 5.6, 5.3, 3.4, 1.75, 1.35, 1.05, 0.86.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Extends from exon 1 into exon 24, from the 5 untranslated region into the 3 untranslated region.
References:

McClatchey AI, et al. The genomic structure of the human skeletal muscle sodium channel gene. Hum. Mol. Genet. 1: 521-527, 1992. PubMed: 1339144

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