Keratinocyte Growth Kit

规格:

货期:

编号:TS171271

品牌:Testobio

产品名称：

Keratinocyte Growth Kit

商品货号：

TS171271

Biosafety Level：

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format：

frozen 1 Kit

Storage Conditions：

-20°C (or -70°C for long-term storage)

Comments：

The Keratinocyte Growth Kit contains components that when added to Dermal Cell Basal Medium (ATCC PCS-200-030) create a complete serum-free culture environment for keratinocytes. The complete keratinocyte growth medium is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation. No feeder layers, extracellular matrix proteins or other substrates are required. The final concentration for each component in complete keratinocyte growth medium is as follows:

Extract P: 0.4%
rh TGF-alpha: 0.5 ng/mL
L-Glutamine: 6 mM
Hydrocortisone: 100 ng/mL
Insulin: 5 µg/mL
Epinephrine: 1.0 µM
Apo-transferrin: 5 µg/mL

Complete Growth Medium：

Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
Obtain one bottle of Dermal Cell Basal Medium (485 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each.

Table 1. Keratinocyte Growth Kit Componentsxa0

Component
Volume：	Volume
Sterility Tests：	Final Concentration
Functional Tests：	Extract P
C of A：	2.0 mL

序号	文章标题	操作
1	ATCC细胞运输和处理	查看详情
2	其他海外品牌代理	查看详情
3	ATCC代理	查看详情
4	购买须知	查看详情
5	订购流程	查看详情

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Keratinocyte Growth Kit

货号: TS171271
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品牌 : TESTOBIO

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产品名称：

Keratinocyte Growth Kit

商品货号：

TS171271

Biosafety Level：

Product Format：

frozen 1 Kit

Storage Conditions：

-20°C (or -70°C for long-term storage)

Comments：

Extract P: 0.4%
rh TGF-alpha: 0.5 ng/mL
L-Glutamine: 6 mM
Hydrocortisone: 100 ng/mL
Insulin: 5 µg/mL
Epinephrine: 1.0 µM
Apo-transferrin: 5 µg/mL

Complete Growth Medium：

Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
Obtain one bottle of Dermal Cell Basal Medium (485 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each.

Table 1. Keratinocyte Growth Kit Componentsxa0