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HFBCT78
HFBCT78
规格:
货期:
编号:TS171440
品牌:Testobio
产品名称: HFBCT78
商品货号: TS171440
Designations: HFBCT78
GenBank Number:

T05242

Species: Homo sapiens, human
Depositors: JC Venter
Vector:
Construct size (kb): 3.799999952316284
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PLA2G2A
Other gene symbols: PLA2L; PLA2B
Gene name: phospholipase A2, group IIA (platelets, synovial fluid)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M86400; EST: EST03131
Nucleotides 1-193 of the insert correspond to
nucleotides 2581-2388 of M86400 with 100% identity.
DNA Seq. Acc.: T05242; EST: EST03131; Primer: M13-21
Insert lengths(kb): 0.800000011920929
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: phospholipase A2, group IIA (platelets, synovial fluid)(phospholipase A2 polypeptide B (synovial fluid)) PLA2B
Insert Size (kb): 0.800
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8.
Based on the insert size and the match to the published sequence, probably a partial clone consisting primarily of 3 untranslated region, because the coding sequence of M86400 is nt 85-822.
References:

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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HFBCT78

  • 货号: TS171440
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: HFBCT78
商品货号: TS171440
Designations: HFBCT78
GenBank Number:

T05242

Species: Homo sapiens, human
Depositors: JC Venter
Vector:
Construct size (kb): 3.799999952316284
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PLA2G2A
Other gene symbols: PLA2L; PLA2B
Gene name: phospholipase A2, group IIA (platelets, synovial fluid)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M86400; EST: EST03131
Nucleotides 1-193 of the insert correspond to
nucleotides 2581-2388 of M86400 with 100% identity.
DNA Seq. Acc.: T05242; EST: EST03131; Primer: M13-21
Insert lengths(kb): 0.800000011920929
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: phospholipase A2, group IIA (platelets, synovial fluid)(phospholipase A2 polypeptide B (synovial fluid)) PLA2B
Insert Size (kb): 0.800
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8.
Based on the insert size and the match to the published sequence, probably a partial clone consisting primarily of 3 untranslated region, because the coding sequence of M86400 is nt 85-822.
References:

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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