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50f2
50f2
规格:
货期:
编号:TS171464
品牌:Testobio
产品名称: 50f2
商品货号: TS171464
Designations: 50f2
Species: Homo sapiens, human
Depositors: J Weissenbach
Vector:
Construct size (kb): 14.39999961853027
Insert:
DNA: genomic
Insert lengths(kb): 1.700000047683716
Gene product: DNA Segment, numerous copies DYS7
Insert Size (kb): 1.700
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.7, 1.7, 1.7, 1.7, 1.7, 1.7; HindIII--3.3, 1.7, 1.7, 1.7, 1.7, 1.7, 1.7; EcoRI/HindIII--3.3, 1.7, 1.7, 1.7, 1.7, 1.7, 1.7; BamHI--14.4; PstI--14.4.
EcoRI detects a 2.4 kb autosomal band and 5 Y-specific bands of 10 kb (A, maps to short arm), 7.5 kb (B, short arm), 6 kb (C, long arm), 4.5 kb (D, centromeric) and 1.7 kb (E, long arm).
TaqI detects autosomal bands of 11 to 12 kb and 10.5 kb, and 4 Y-specific bands of 9 kb (equivalent to EcoRI band E), 8 kb (EcoRI band D), 3.5 kb (EcoRI band A or B) and 2.8 kb (two fragments, equivalent to EcoRI C and EcoRI A or B).
Plasmid contains 6 copies of the 1.7 kb insert.
Wash at high stringency (0.1X SSC, 68C).
References:

Vergnaud G, et al. A deletion map of the human Y chromosome based on DNA hybridization. Am. J. Hum. Genet. 38: 109-124, 1986. PubMed: 3004206

Jean Weissenbach, personal communication

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50f2

  • 货号: TS171464
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: 50f2
商品货号: TS171464
Designations: 50f2
Species: Homo sapiens, human
Depositors: J Weissenbach
Vector:
Construct size (kb): 14.39999961853027
Insert:
DNA: genomic
Insert lengths(kb): 1.700000047683716
Gene product: DNA Segment, numerous copies DYS7
Insert Size (kb): 1.700
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.7, 1.7, 1.7, 1.7, 1.7, 1.7; HindIII--3.3, 1.7, 1.7, 1.7, 1.7, 1.7, 1.7; EcoRI/HindIII--3.3, 1.7, 1.7, 1.7, 1.7, 1.7, 1.7; BamHI--14.4; PstI--14.4.
EcoRI detects a 2.4 kb autosomal band and 5 Y-specific bands of 10 kb (A, maps to short arm), 7.5 kb (B, short arm), 6 kb (C, long arm), 4.5 kb (D, centromeric) and 1.7 kb (E, long arm).
TaqI detects autosomal bands of 11 to 12 kb and 10.5 kb, and 4 Y-specific bands of 9 kb (equivalent to EcoRI band E), 8 kb (EcoRI band D), 3.5 kb (EcoRI band A or B) and 2.8 kb (two fragments, equivalent to EcoRI C and EcoRI A or B).
Plasmid contains 6 copies of the 1.7 kb insert.
Wash at high stringency (0.1X SSC, 68C).
References:

Vergnaud G, et al. A deletion map of the human Y chromosome based on DNA hybridization. Am. J. Hum. Genet. 38: 109-124, 1986. PubMed: 3004206

Jean Weissenbach, personal communication

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